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Abstract：
In order to achieve continuous industrial production of astaxanthin in Xanthophyllomyces dendrorhous, a moderate temperature (25-37°C) fermentation process was needed. In this study, a two-step process with a 20°C pre-culture for 18 h and a 30°C culture for 30 h was performed to achieve the astaxanthin yields of 116.42 μg g-1 dry cell weight, which was lower than that in the normal process (20°C, 96 h). However, cell yield (YX/S) and product yield (YP/S) showed no significant differences between the two processes, suggesting that moderate temperature did not affect the productivity of astaxanthin. The transcriptional levels of genes involved in astaxanthin synthesis were compared in different culture times and a negative correlation between temperature and expression of carotenogenic genes was found. This work provided a potential method for continuous production of astaxanthin using X. dendrorhous at moderate temperature throughout the year.
摘要：
为了实现枝状叶黄素中虾青素的连续工业化生产,需要中等温度(25-37°C)的发酵过程。在这项研究中,进行20°C预培养18小时和30°C培养30小时的两步过程，以实现虾青素产量116.42μgg-1干细胞重量，低于正常过程中的温度(20℃，96小时)。然而,细胞产量(YX/S)和产物产量(YP/S)在两个过程中没有显著差异,表明适度的温度不会影响虾青素的生产率。比较了不同培养时间中虾青素合成相关基因的转录水平，发现温度与类胡萝卜素基因的表达呈负相关。这项工作提供了一种使用X连续生产虾青素的潜在方法。全年在中等温度下的树枝状。
