缩短 sgRNA - DNA 界面使得 SpCas9 和 eSpCas9 (1.1) 能够在靶 DNA 链上产生切口。
关键词: DSB SpCas9 co-editing eSpCas9(1.1) nickase truncated spacer
Mesh : Base Sequence CRISPR-Associated Protein 9 / genetics metabolism CRISPR-Cas Systems DNA / chemistry metabolism DNA Breaks, Double-Stranded Deoxyribonuclease I / genetics metabolism Gene Editing Mutation Streptococcus pyogenes / enzymology RNA, Guide, CRISPR-Cas Systems
来 源:
DOI:10.1007/s11427-020-1722-0
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