Abstract：
OBJECTIVE: The effects of three preoperative mouthwashes on salivary bacterial levels were evaluated and compared between subjects with differing periodontal status.
METHODS: Based on periodontal parameters, periodontally healthy individuals (n = 60) and those with gingivitis (n = 60) and periodontitis (n = 60) were randomly assigned to a single preoperative dose of chlorhexidine (CHX), essential oils (EO), cetylpyridinium chloride (CPC) or negative control mouthwashes. Saliva samples were collected between 8:00 and 11:00 a.m., before and after a single-dose rinse with the respective mouthwash. Total bacterial load and levels of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Streptococcus oralis were determined by qPCR. Data were statistically analysed using paired t- and Student\'s t-tests (p < 0.05).
RESULTS: CHX, EO and CPC showed greater antimicrobial efficacy than did the negative control. CHX [1226445.53] and EO [1743639.38] provided greater reductions in comparison to both CPC [106302.96] and negative control [37852.46]). CHX provided greater reductions of simultaneous levels of Pg [106326.00], Td [3335841] and Tf [61557.47] in the healthy group, as did EO in the diseased groups. CPC provided the greatest reduction [3775319.36] in the periodontitis group.
CONCLUSIONS: Periodontal status influenced the antimicrobial efficacy of preoperative mouthwashes. Therefore, periodontal status should be taken into consideration by clinicians. The antimicrobial efficacy differed among the agents tested. CHX and EO showed the greatest efficacy. The recognition of periodontal condition by clinicians is mandatory to select the most effective preoperative mouthwash.
METHODS: Based on periodontal parameters, periodontally healthy individuals (n = 60) and those with gingivitis (n = 60) and periodontitis (n = 60) were randomly assigned to a single preoperative dose of chlorhexidine (CHX), essential oils (EO), cetylpyridinium chloride (CPC) or negative control mouthwashes. Saliva samples were collected between 8:00 and 11:00 a.m., before and after a single-dose rinse with the respective mouthwash. Total bacterial load and levels of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Streptococcus oralis were determined by qPCR. Data were statistically analysed using paired t- and Student\'s t-tests (p < 0.05).
RESULTS: CHX, EO and CPC showed greater antimicrobial efficacy than did the negative control. CHX [1226445.53] and EO [1743639.38] provided greater reductions in comparison to both CPC [106302.96] and negative control [37852.46]). CHX provided greater reductions of simultaneous levels of Pg [106326.00], Td [3335841] and Tf [61557.47] in the healthy group, as did EO in the diseased groups. CPC provided the greatest reduction [3775319.36] in the periodontitis group.
CONCLUSIONS: Periodontal status influenced the antimicrobial efficacy of preoperative mouthwashes. Therefore, periodontal status should be taken into consideration by clinicians. The antimicrobial efficacy differed among the agents tested. CHX and EO showed the greatest efficacy. The recognition of periodontal condition by clinicians is mandatory to select the most effective preoperative mouthwash.
摘要：
目的：评估三种术前漱口水对唾液细菌水平的影响，并在不同牙周状态的受试者之间进行比较。
方法：根据牙周参数，牙周健康个体（n=60）和牙龈炎（n=60）和牙周炎（n=60）被随机分配到单剂量的术前氯己定（CHX），精油（EO），氯化十六烷基吡啶(CPC)或阴性对照漱口水。在上午8:00到11:00之间收集唾液样本，在用各自的漱口水单剂量冲洗之前和之后。牙龈卟啉单胞菌的总细菌载量和水平，连翘坦菌,通过qPCR测定了树突状螺旋体和口腔链球菌。使用配对t检验和Studentt检验(p<0.05)对数据进行统计学分析。
结果：CHX，EO和CPC显示出比阴性对照更大的抗微生物效力。与CPC[106302.96]和阴性对照[37852.46]相比,CHX[1226445.53]和EO[1743639.38]提供更大的减少)。CHX提供了更大的同时Pg水平降低[106326.00]，健康组的Td[3335841]和Tf[61557.47]，EO在患病组中也是如此。CPC在牙周炎组中提供了最大的减少[3775319.36]。
结论：牙周状态影响术前漱口水的抗菌效果。因此,临床医生应考虑牙周状况。所测试的试剂之间的抗微生物功效不同。CHX和EO显示出最大的功效。临床医生必须识别牙周状况，以选择最有效的术前漱口水。
方法：根据牙周参数，牙周健康个体（n=60）和牙龈炎（n=60）和牙周炎（n=60）被随机分配到单剂量的术前氯己定（CHX），精油（EO），氯化十六烷基吡啶(CPC)或阴性对照漱口水。在上午8:00到11:00之间收集唾液样本，在用各自的漱口水单剂量冲洗之前和之后。牙龈卟啉单胞菌的总细菌载量和水平，连翘坦菌,通过qPCR测定了树突状螺旋体和口腔链球菌。使用配对t检验和Studentt检验(p<0.05)对数据进行统计学分析。
结果：CHX，EO和CPC显示出比阴性对照更大的抗微生物效力。与CPC[106302.96]和阴性对照[37852.46]相比,CHX[1226445.53]和EO[1743639.38]提供更大的减少)。CHX提供了更大的同时Pg水平降低[106326.00]，健康组的Td[3335841]和Tf[61557.47]，EO在患病组中也是如此。CPC在牙周炎组中提供了最大的减少[3775319.36]。
结论：牙周状态影响术前漱口水的抗菌效果。因此,临床医生应考虑牙周状况。所测试的试剂之间的抗微生物功效不同。CHX和EO显示出最大的功效。临床医生必须识别牙周状况，以选择最有效的术前漱口水。
