Investigation of an imported cholera case in China with whole genome sequencing.

• 影响指数: 4.393
• 发表时间: 10 2020
• 来源期刊: Infect Genet Evol
• DOI: 10.1016/j.meegid.2020.104362
• PMID: 32422352
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 医学
• 作者列表: Li F,Pang B,Yan H,Lu X,Li J,Zhou H,Cui Z,Zhao L,Mahemut ,Huo D,Kan B,Jia L

关键词: Imported cholera case; Phylogenetic analysis; Vibrio cholerae; Whole genome sequencing

Mesh: Adult; China; Cholera / etiology microbiology; Female; Genome, Bacterial; Humans; India; Nepal; Phylogeny; Vibrio cholerae O1 / genetics isolation & purification pathogenicity; Whole Genome Sequencing

Abstract

Determining the source and genetic characteristics of the imported pathogen is critical in the control of infectious diseases. Here, we reported the investigation of an imported cholera case in China in 2018 with a recent travel history in Nepal and India. Stool culture from the patient was identified as Vibrio cholerae serogroup O1, biotype El Tor, serotype Ogawa. The strain 2018HL24 possessed intact Vibrio seventh pandemic island I (VSP-I), Vibrio pathogenicity Island 1 and 2 (VPI-1, VPI-2). A VSP-II variant with a 13 kb deletion was also detected, which was identical to those observed in V. cholerae in cluster \"Nepal-4\". Phylogenetic analysis based on the core genome SNPs showed that the isolate was most closely related to the V. cholerae isolated in northern India not far from the border of Nepal in 2012 (16 SNPs). Combining the epidemiological data with phylogenetic analysis results, we speculate that the patient may got infected in Nepal-India region.