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Abstract：
Detection of animal materials in gelatin-based products is required to address religious and cultural concerns, because porcine and bovine gelatins are prohibited in Halal, Kosher and Hindus consumer goods. In this paper, multiplex quantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discriminate bovine, porcine and fish gelatin species in a single assay platform. The assay was specific to cattle, pigs and fish, having been tested against 14 non-target species. The limit of detection, under gelatin admixed conditions, was 0.005 ng/µL. Finally, a pilot survey was undertaken testing 35 Halal branded processed food and dietary items. Out of 35 samples, only two were found to be positive for porcine species. The authenticity of these two qPCR products was confirmed by DNA sequencing analysis, which showed 99-100% similarity with Sus scrofa (Wild boar) species.
摘要：
为了解决宗教和文化问题，需要检测明胶产品中的动物材料，因为清真中禁止猪和牛明胶，犹太和印度教徒的消费品。在本文中,使用TaqMan探针的多重定量聚合酶链反应（qPCR）测定法被开发用于区分牛，在单个测定平台中的猪和鱼明胶物种。该试验是针对牛的，猪和鱼,已经针对14种非目标物种进行了测试。检测限，在明胶混合条件下，为0.005ng/µL。最后,进行了一项试点调查，测试了35种清真品牌加工食品和膳食产品。在35个样本中，只有两个被发现是阳性的猪种。通过DNA测序分析证实了这两种qPCR产物的真实性，与Susscrofa（野猪）物种具有99-100％的相似性。
