关键词: Autographivirinae O-specific polysaccharide Pectobacterium carotovorum bacteriophage lipopolysaccharide random sugar O-acetylation rhamnosidase tail spike protein taxonomy

Mesh : Amino Acid Sequence Bacteriophages / physiology ultrastructure Genome, Viral Genomics Magnetic Resonance Spectroscopy Models, Molecular Molecular Conformation Pectobacterium carotovorum / metabolism virology Phylogeny Polymerization Polysaccharides, Bacterial / chemistry Protein Binding Viral Proteins / chemistry

来  源:   DOI:10.3390/ijms21093170   PDF(Sci-hub)   PDF(Pubmed)

Abstract:
Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of numerous amendments in the taxonomy of P. carotovorum, there are still a few studied sequenced strains among this species. The present work reports on the isolation and characterization of the phage infectious to the type strain of P. carotovorum. The phage Arno 160 is a lytic Podovirus representing a potential new genus of the subfamily Autographivirinae. It recognizes O-polysaccahride of the host strain and depolymerizes it in the process of infection using a rhamnosidase hydrolytic mechanism. Despite the narrow host range of this phage, it is suitable for phage control application.
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