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Abstract：
Horse testis development and spermatogenesis are complex physiological processes.
To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis.
A total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to \"spermatogenesis,\" male gamete generation,\" \"spermatid development\" and \"oocyte meiosis\" were significantly involved in different stages of testis development and spermatogenesis.
Genes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis.
To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis.
A total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to \"spermatogenesis,\" male gamete generation,\" \"spermatid development\" and \"oocyte meiosis\" were significantly involved in different stages of testis development and spermatogenesis.
Genes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis.
摘要：
马睾丸发育和精子发生是复杂的生理过程。
为了研究这些过程，从蒙古马身上收集了三个未成熟和三个成熟的睾丸，并利用高通量RNA测序技术(RNA-Seq)建立6个文库,筛选与睾丸发育和精子发生相关的基因。
在蒙古马的睾丸中检测到总共16,237个上调基因和8,641个下调基因。这些基因在精子发生和睾丸发育的不同发育阶段起着重要作用。检测到五个具有选择性剪接事件的基因，这些基因可能会影响精子发生和睾丸发育。进行GO(基因本体论)和KEGG(京都基因和基因组百科全书)途径分析以用于差异表达基因的功能注释。与精子发生有关的途径，“雄性配子一代，“精子细胞发育”和“卵母细胞减数分裂”在睾丸发育和精子发生的不同阶段均显着参与。
基因,通过推断蒙古马精子发生过程中的功能，确定了途径和可变剪接事件。这些差异表达的遗传特征的鉴定提高了我们对马睾丸发育和精子发生的理解。
为了研究这些过程，从蒙古马身上收集了三个未成熟和三个成熟的睾丸，并利用高通量RNA测序技术(RNA-Seq)建立6个文库,筛选与睾丸发育和精子发生相关的基因。
在蒙古马的睾丸中检测到总共16,237个上调基因和8,641个下调基因。这些基因在精子发生和睾丸发育的不同发育阶段起着重要作用。检测到五个具有选择性剪接事件的基因，这些基因可能会影响精子发生和睾丸发育。进行GO(基因本体论)和KEGG(京都基因和基因组百科全书)途径分析以用于差异表达基因的功能注释。与精子发生有关的途径，“雄性配子一代，“精子细胞发育”和“卵母细胞减数分裂”在睾丸发育和精子发生的不同阶段均显着参与。
基因,通过推断蒙古马精子发生过程中的功能，确定了途径和可变剪接事件。这些差异表达的遗传特征的鉴定提高了我们对马睾丸发育和精子发生的理解。
