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Abstract：
Cotton possesses certain physical features, including leaf and stem trichomes that help plants deter damage caused by insect pests, and to some extent, from abiotic factors as well. Among those features, trichomes (pubescence) hold a special place as a first line of defense and a managemental tool against sucking insect pests of cotton. Different insect pests of cotton (whiteflies, aphids, jassids, and boll weevil) severely damage the yield and quality of the crop. Likewise, whiteflies, aphids, jassids, and other insect pests are considered as potential carriers for cotton leaf curl viruses and other diseases. Genotyping by sequencing (GBS) study was conducted to understand and explore the genomic regions governing hairy (Pubescence) leaves and stem phenotypes. A total of 224 individuals developed from an intraspecific cross (densely haired cotton (Liaoyang duomao mian) × hairless cotton (Zong 128)) and characterized phenotypically for leaf and stem pubescence in different environments. Here we identify and report significant QTLs (quantitative trait loci) associated with leaf and stem pubescence, and the response of plant under pest (aphid) infestation. Further, we identified putative genes colocalized on chromosome A06 governing mechanism for trichome development and host-pest interaction. Our study provides a comprehensive insight into genetic architecture that can be employed to improve molecular marker-assisted breeding programs aimed at developing biotic (insect pests) resilient cotton cultivars.
摘要：
棉花具有一定的物理特性,包括叶和茎毛状体，帮助植物阻止害虫造成的损害，在某种程度上,也来自非生物因素。在这些特征中，毛状体（柔毛）作为第一道防线和对抗棉花吸虫害虫的管理工具具有特殊的位置。棉花的不同害虫（粉虱，蚜虫,Jassids,和棉铃象鼻虫）严重损害作物的产量和质量。同样，粉虱,蚜虫,Jassids,和其他害虫被认为是棉花曲叶病毒和其他疾病的潜在载体。通过测序进行基因分型(GBS)研究以理解和探索控制毛状(毛状)叶和茎表型的基因组区域。从种内杂交（密毛棉（辽阳多毛棉）×无毛棉（Zong128））中发育出总共224个个体，并在不同环境中对叶片和茎的青春期进行了表型表征。在这里，我们确定和报告显著的QTL（数量性状位点）相关的叶和茎短柔毛，以及植物在害虫(蚜虫)侵染下的反应。Further,我们确定了共定位于A06染色体上的推定基因，其控制毛状体发育和宿主-害虫相互作用的机制。我们的研究提供了对遗传结构的全面了解，可用于改善旨在开发生物（害虫）弹性棉花品种的分子标记辅助育种计划。
