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Abstract：
BACKGROUND: We have been trying to produce scaffold-free structures for airway regeneration using a bio-3D-printer with spheroids, to avoid scaffold-associated risks such as infection. Previous studies have shown that human umbilical vein endothelial cells (HUVECs) play an important role in such structures, but HUVECs cannot be isolated from adult humans. The aim of this study was to identify alternatives to HUVECs for use in scaffold-free structures.
METHODS: Three types of structure were compared, made of chondrocytes and mesenchymal stem cells with HUVECs, human lung microvascular endothelial cells (HMVEC-Ls), and induced pluripotent stem cell (iPSC)-derived endothelial cells.
RESULTS: No significant difference in tensile strength was observed between the three groups. Histologically, some small capillary-like tube formations comprising CD31-positive cells were observed in all groups. The number and diameters of such formations were significantly lower in the iPSC-derived endothelial cell group than in other groups. Glycosaminoglycan content was significantly lower in the iPSC-derived endothelial cell group than in the HUVEC group, while no significant difference was observed between the HUVEC and HMVEC-L groups.
CONCLUSIONS: HMVEC-Ls can replace HUVECs as a cell source for scaffold-free trachea-like structures. However, some limitations were associated with iPSC-derived endothelial cells.
METHODS: Three types of structure were compared, made of chondrocytes and mesenchymal stem cells with HUVECs, human lung microvascular endothelial cells (HMVEC-Ls), and induced pluripotent stem cell (iPSC)-derived endothelial cells.
RESULTS: No significant difference in tensile strength was observed between the three groups. Histologically, some small capillary-like tube formations comprising CD31-positive cells were observed in all groups. The number and diameters of such formations were significantly lower in the iPSC-derived endothelial cell group than in other groups. Glycosaminoglycan content was significantly lower in the iPSC-derived endothelial cell group than in the HUVEC group, while no significant difference was observed between the HUVEC and HMVEC-L groups.
CONCLUSIONS: HMVEC-Ls can replace HUVECs as a cell source for scaffold-free trachea-like structures. However, some limitations were associated with iPSC-derived endothelial cells.
摘要：
背景：我们一直在尝试使用带有球体的生物3D打印机生产用于气道再生的无支架结构，以避免与支架相关的风险，如感染。先前的研究表明，人脐静脉内皮细胞（HUVECs）在此类结构中起着重要作用，但是HUVECs不能与成年人分离。这项研究的目的是确定用于无支架结构的HUVEC的替代品。
方法：比较了三种结构类型，由软骨细胞和间充质干细胞与HUVECs，人肺微血管内皮细胞（HMVEC-Ls），和诱导多能干细胞（iPSC）衍生的内皮细胞。
结果：三组之间的拉伸强度没有显着差异。组织学上,在所有组中都观察到一些包含CD31阳性细胞的小毛细管样导管形成.在iPSC衍生的内皮细胞组中，这种形成的数量和直径明显低于其他组。在iPSC来源的内皮细胞组中糖胺聚糖含量显著低于HUVEC组,而HUVEC和HMVEC-L组之间没有观察到显著差异。
结论：HMVEC-Ls可以替代HUVEC作为无支架气管样结构的细胞来源。然而,一些局限性与iPSC来源的内皮细胞相关.
方法：比较了三种结构类型，由软骨细胞和间充质干细胞与HUVECs，人肺微血管内皮细胞（HMVEC-Ls），和诱导多能干细胞（iPSC）衍生的内皮细胞。
结果：三组之间的拉伸强度没有显着差异。组织学上,在所有组中都观察到一些包含CD31阳性细胞的小毛细管样导管形成.在iPSC衍生的内皮细胞组中，这种形成的数量和直径明显低于其他组。在iPSC来源的内皮细胞组中糖胺聚糖含量显著低于HUVEC组,而HUVEC和HMVEC-L组之间没有观察到显著差异。
结论：HMVEC-Ls可以替代HUVEC作为无支架气管样结构的细胞来源。然而,一些局限性与iPSC来源的内皮细胞相关.
