METHODS: Three types of structure were compared, made of chondrocytes and mesenchymal stem cells with HUVECs, human lung microvascular endothelial cells (HMVEC-Ls), and induced pluripotent stem cell (iPSC)-derived endothelial cells.
RESULTS: No significant difference in tensile strength was observed between the three groups. Histologically, some small capillary-like tube formations comprising CD31-positive cells were observed in all groups. The number and diameters of such formations were significantly lower in the iPSC-derived endothelial cell group than in other groups. Glycosaminoglycan content was significantly lower in the iPSC-derived endothelial cell group than in the HUVEC group, while no significant difference was observed between the HUVEC and HMVEC-L groups.
CONCLUSIONS: HMVEC-Ls can replace HUVECs as a cell source for scaffold-free trachea-like structures. However, some limitations were associated with iPSC-derived endothelial cells.
方法:比较了三种结构类型,由软骨细胞和间充质干细胞与HUVECs,人肺微血管内皮细胞(HMVEC-Ls),和诱导多能干细胞(iPSC)衍生的内皮细胞。
结果:三组之间的拉伸强度没有显着差异。组织学上,在所有组中都观察到一些包含CD31阳性细胞的小毛细管样导管形成.在iPSC衍生的内皮细胞组中,这种形成的数量和直径明显低于其他组。在iPSC来源的内皮细胞组中糖胺聚糖含量显著低于HUVEC组,而HUVEC和HMVEC-L组之间没有观察到显著差异。
结论:HMVEC-Ls可以替代HUVEC作为无支架气管样结构的细胞来源。然而,一些局限性与iPSC来源的内皮细胞相关.