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Abstract：
Changes in glycoprotein content, altered glycosylations, and aberrant glycan structures are increasingly recognized as cancer hallmarks. Because breast cancer is one of the most common causes of cancer deaths in the world, it is highly urgent to find other reliable biomarkers for its initial diagnosis and to learn as much as possible about this disease. In this Review, the applications of lectins to a screening of potential breast cancer biomarkers published during recent years are overviewed. These data provide a deeper insight into the use of modern strategies, technologies, and scientific knowledge in glycoproteomic breast cancer research. Particular attention is concentrated on the use of lectin-based affinity techniques, applied independently or most frequently in combination with mass spectrometry, as an effective tool for the targeting, separation, and reliable identification of glycoprotein molecules. Individual procedures and lectins used in published glycoproteomic studies of breast-cancer-related glycoproteins are discussed. The summarized approaches have the potential for use in diagnostic and predictive applications. Finally, the use of lectins is briefly discussed from the view of their future applications in the analysis of glycoproteins in cancer.
摘要：
糖蛋白含量的变化，糖基化改变，和异常的聚糖结构越来越被认为是癌症的标志。因为乳腺癌是世界上最常见的癌症死亡原因之一，迫切需要找到其他可靠的生物标志物来进行初步诊断，并尽可能多地了解这种疾病。在这篇评论中,概述了近年来发表的凝集素在潜在乳腺癌生物标志物筛查中的应用。这些数据为现代策略的使用提供了更深入的见解，技术,和乳腺癌糖蛋白组学研究的科学知识。特别注意集中在使用基于凝集素的亲和技术，独立应用或最频繁地与质谱联用，作为目标的有效工具，分离,和可靠的糖蛋白分子鉴定。讨论了已发表的乳腺癌相关糖蛋白的糖蛋白质组学研究中使用的各个程序和凝集素。总结的方法具有用于诊断和预测应用的潜力。最后,从凝集素在癌症糖蛋白分析中的未来应用的角度，简要讨论了凝集素的用途。
