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Abstract：
Bisphenol A diglycidyl ether (BADGE) is an epoxy resin used for the inner coating of canned food and beverages. BADGE can easily migrate from the containers and become a contaminant. In this study, we examined the effects of BADGE exposure to the dams on the behavioral, structural, and developmental abnormalities in the offspring. Female pregnant mice were fed with a diet containing BADGE (0.15 or 1.5 mg/kg/day) during gestation and lactation periods. In an open field test, the time spent in the corner area significantly increases in male mice of high-dose BADGE group at 5 weeks old. The histological analysis using offspring brain at postnatal day 1 delivered from BADGE (1.5 mg/kg/day)-treated dams demonstrates that positive signals of Forkhead box P2- and COUP-TF interacting protein 2 are restricted in each cortical layer, but not in the control brain. In addition, the maternal BADGE exposure reduces nestin-positive fibers of the radial glia and T-box transcription factor 2-positive intermediate progenitors in the inner subventricular zone. Furthermore, a direct BADGE exposure promotes neurite outgrowth and neuronal connection in the primary cultured cortical neurons. These data suggest that maternal BADGE exposure can accelerate neuronal differentiation in fetuses and induce anxiety-like behavior in juvenile mice.
摘要：
双酚A二缩水甘油醚(BADGE)是用于罐头食品和饮料的内涂层的环氧树脂。BADGE很容易从容器中迁移并成为污染物。在这项研究中,我们研究了BADGE暴露于水坝对行为的影响，结构,和后代的发育异常。在妊娠和哺乳期期间,用含有BADGE(0.15或1.5mg/kg/天)的饮食喂养雌性怀孕小鼠。在露天测试中，高剂量BADGE组雄性小鼠在5周龄时在角区花费的时间显著增加.在出生后第1天，从BADGE（1.5mg/kg/天）处理的水坝中使用后代大脑进行的组织学分析表明，叉头盒P2-和COUP-TF相互作用蛋白2的阳性信号在每个皮质层中受到限制层，但不在控制大脑中。此外,母体BADGE暴露减少了radial神经胶质细胞的nestin阳性纤维和内心室下区的T-box转录因子2阳性中间祖细胞。此外，BADGE直接暴露可促进原代培养的皮质神经元的神经突生长和神经元连接。这些数据表明，母体BADGE暴露可以加速胎儿的神经元分化，并在幼年小鼠中诱导焦虑样行为。
