PDF(Sci-hub)
Abstract：
The objective of this pilot study was to assess the fecal microbiome and different immunological parameters in infant feces and maternal milk from mother-infant pairs in which the infants were suffering from different gastrointestinal disorders (colic, non-IgE-mediated cow milk protein allergy (CMPA), and proctocolitis). A cohort of 30 mother-infant pairs, in which the infants were diagnosed with these gastrointestinal disorders or included as healthy controls, were recruited. Bacterial composition of infant feces and breast milk was determined by metataxonomic sequencing. Immunological compounds were quantified using multiplexed immunoassays. A higher abundance of Eggerthellaceae, Lachnospiraceae and Peptostreptococcaceae, and lower abundance of Bifidobacterium and higher abundance of Rothia were registered in fecal samples from the CMPA group. Eggerthellaceae was also significantly more abundant in milk samples of the CMPA group. There were no differences in the concentration of immunological compounds in infant fecal samples between the four groups. In contrast, differences were found in the concentration and/or frequency of compounds related to acquired immunity and granulocyte colony stimulating factor (GCSF) in breast milk samples. In conclusion, a few microbial signatures in feces may explain part of the difference between CMPA and other infants. In addition, some milk immunological signatures have been uncovered among the different conditions addressed in this pilot study.
摘要：
这项初步研究的目的是评估婴儿粪便和母乳中的粪便微生物组和不同的免疫学参数，其中婴儿患有不同的胃肠道疾病（绞痛，非IgE介导的牛奶蛋白过敏（CMPA），和直肠结肠炎）。一组30对母婴，其中婴儿被诊断患有这些胃肠道疾病或作为健康对照，被招募。通过代谢测序确定婴儿粪便和母乳的细菌组成。使用多重免疫测定对免疫化合物进行定量。虫草科的丰度较高，Lachnospiraceae和肽链球菌科，在CMPA组的粪便样本中，双歧杆菌的丰度较低，而Rothia的丰度较高。Eggerthellaceae在CMPA组的牛奶样品中也明显更丰富。四组之间婴儿粪便样品中免疫化合物的浓度没有差异。相比之下,母乳样品中与获得性免疫和粒细胞集落刺激因子(GCSF)相关的化合物的浓度和/或频率存在差异.总之,粪便中的一些微生物特征可以部分解释CMPA与其他婴儿之间的差异.此外,在这项初步研究中，已经发现了一些牛奶免疫特征。
