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Abstract：
Background: LncRNA-ZNF281 suppresses glioma, whereas its role in hepatocellular carcinoma (HCC) is unclear. This study aimed to investigate the interaction between ZNF281 and miR-539 in HCC. Materials and Methods: This study included 66 HCC patients (40 men and 26 women; 36-68 years, 53.1 ± 6.2 years) who were selected from the 133 HCC cases admitted to The First Affiliated Hospital of Fujian Medical University from February 2011 to June 2014. Levels of ZNF281 and miR-539 expression in two types of tissues (HCC and nontumor) were measured by performing quantitative polymerase chain reaction (qPCR). Dual-luciferase assay was performed to analyze the interactions between miR-539 and ZNF281 in both SNU-475 and PLHC-1 cells. The effects of ZNF281 and miR-539 overexpression on the invasion and migration of HCC cells were analyzed by performing transwell assays. Results: The authors showed that ZNF281 was upregulated and miR-539 was downregulated in HCC tissues and were negatively correlated. High levels of ZNF281 and low levels of miR-539 predicted the poor survival of HCC patients. Overexpression analysis showed that ZNF281 and miR-539 overexpression led to the downregulation of each other. Transwell assays showed that ZNF281 overexpression led to enhanced and miR-539 overexpression led to suppressed HCC cell invasion and migration. In addition, miR-539 overexpression attenuated the effects of ZNF281 overexpression. Conclusions: Therefore, ZNF281 may interact with miR-539 to promote HCC cell invasion and migration.
摘要：
背景：LncRNA-ZNF281抑制胶质瘤，而其在肝细胞癌（HCC）中的作用尚不清楚。本研究旨在探讨ZNF281和miR-539在HCC中的相互作用。材料和方法：本研究包括66例HCC患者（40例男性和26例女性；36-68岁，从2011年2月至2014年6月福建医科大学附属第一医院收治的133例HCC患者中选取53.1±6.2岁)。通过进行定量聚合酶链反应（qPCR）测量两种类型的组织（HCC和非肿瘤）中ZNF281和miR-539的表达水平。进行双荧光素酶测定以分析SNU-475和PLHC-1细胞中miR-539和ZNF281之间的相互作用。通过进行transwell测定分析ZNF281和miR-539过表达对HCC细胞侵袭和迁移的影响。结果：作者发现ZNF281上调，miR-539在HCC组织中下调，且呈负相关。高水平的ZNF281和低水平的miR-539预测HCC患者的低生存率。过表达分析显示ZNF281和miR-539过表达导致彼此下调。Transwell分析显示ZNF281过表达导致增强，miR-539过表达导致抑制HCC细胞侵袭和迁移。此外,miR-539过表达减弱了ZNF281过表达的作用。结论：因此，ZNF281可能与miR-539相互作用以促进HCC细胞的侵袭和迁移。
