TMEM16A Ca2 + 激活的 Cl - 通道抑制通过 IP3R / Ca2 + / NF κ B / IL - 6 信号通路改善急性胰腺炎。
关键词: AP, acute pancreatitis Acute pancreatitis BAPTA-AM, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid-acetyloxymethyl ester CCK, cholesystokinin CFBE, cystic fibrosis bronchial epithelial CaCCinh-A01, Ca2+-activated Cl− channel inhibitor-A01 EDTA, ethylenediaminetetraacetic acid EGF, epidermal growth factor EGFP, green fluorescent protein EGFR, epidermal growth factor receptor EGTA, ethylene glycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid ELISA, enzyme-linked immunosorbent assay ER, endoplasmic reticulum FBS, fetal bovine serum HEPES, N-2-hydroxyethil-piperazine-N'-2-ethanesulfonic acid IL-6, interleukin 6 IL-6R, interleukin 6 receptor IP3R, inositol 1,4,5-trisphosphate receptor Inositol 1,4,5-trisphosphate receptor Interleukin-6 NFκB NFκB, nuclear factor-κB NMDG, N-methyl-D-glucamine NP-40, Nonidet P-40 PACs, pancreatic acinar cells RIPA, radio immunoprecipitation assay SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis STAT3, signal transducers and activators of transcription 3 T16Ainh-A01, TMEM16A inhibitor-A01 TMEM16A Tris, tris(hydroxymethyl)aminomethane WT, wild type shRNAs, short hairpin RNAs
来 源:
DOI:10.1016/j.jare.2020.01.006
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