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Abstract：
BACKGROUND: Head-twitch response (HTR) is a manifestation of the serotonergic system behavioral pharmacology commonly used as a proxy of psychedelic drug action in rodents.
METHODS: We developed a minimally invasive magnetic ear tag reporter and designed a detection system that performs a comprehensive characterization of each potential HTR event on an electromagnetic readout.
RESULTS: Magnetic ear tags were easy to install and generally well tolerated by the animals. On the low-threshold first phase of detection, the tags\' signal recorded in a magnetometer was filtered and screened for potential HTR events. On the second phase, the detector performed a comprehensive spectral analysis evaluation of each event and identified the HTR characteristic distribution of power density. Our system delivered satisfactory performance in the identification of pharmacologically-induced HTR and discrimination power against common non-HTR behaviors.
METHODS: Our system offers a high-throughput solution for studying HTR in mice employing minimally invasive procedures and superior standalone discriminative power compared to our previously reported fully-automated approach.
CONCLUSIONS: High-throughput identification of HTR utilizing magnetic ear-tagging and biphasic detection delivers satisfactory detection and discrimination power employing less invasive procedures.
METHODS: We developed a minimally invasive magnetic ear tag reporter and designed a detection system that performs a comprehensive characterization of each potential HTR event on an electromagnetic readout.
RESULTS: Magnetic ear tags were easy to install and generally well tolerated by the animals. On the low-threshold first phase of detection, the tags\' signal recorded in a magnetometer was filtered and screened for potential HTR events. On the second phase, the detector performed a comprehensive spectral analysis evaluation of each event and identified the HTR characteristic distribution of power density. Our system delivered satisfactory performance in the identification of pharmacologically-induced HTR and discrimination power against common non-HTR behaviors.
METHODS: Our system offers a high-throughput solution for studying HTR in mice employing minimally invasive procedures and superior standalone discriminative power compared to our previously reported fully-automated approach.
CONCLUSIONS: High-throughput identification of HTR utilizing magnetic ear-tagging and biphasic detection delivers satisfactory detection and discrimination power employing less invasive procedures.
摘要：
背景：头部抽搐反应（HTR）是5-羟色胺能系统行为药理学的一种表现，通常用作啮齿类动物中迷幻药作用的代理。
方法：我们开发了一种微创磁性耳标报告器，并设计了一种检测系统，该系统在电磁读出器上对每个潜在的HTR事件进行全面表征。
结果:磁性耳标易于安装并且通常被动物良好耐受。在低阈值检测的第一阶段，对磁力计中记录的标记信号进行过滤并筛选潜在的HTR事件。在第二阶段,探测器对每个事件进行了全面的频谱分析评估，并确定了功率密度的HTR特征分布。我们的系统在识别药理学诱导的HTR和对常见非HTR行为的辨别能力方面提供了令人满意的性能。
方法：与我们之前报道的全自动方法相比，我们的系统为采用微创程序和卓越的独立鉴别能力在小鼠中研究HTR提供了高通量解决方案。
结论：利用磁耳标记和双相检测的HTR的高通量鉴定，采用侵入性较小的程序可提供令人满意的检测和辨别能力。
方法：我们开发了一种微创磁性耳标报告器，并设计了一种检测系统，该系统在电磁读出器上对每个潜在的HTR事件进行全面表征。
结果:磁性耳标易于安装并且通常被动物良好耐受。在低阈值检测的第一阶段，对磁力计中记录的标记信号进行过滤并筛选潜在的HTR事件。在第二阶段,探测器对每个事件进行了全面的频谱分析评估，并确定了功率密度的HTR特征分布。我们的系统在识别药理学诱导的HTR和对常见非HTR行为的辨别能力方面提供了令人满意的性能。
方法：与我们之前报道的全自动方法相比，我们的系统为采用微创程序和卓越的独立鉴别能力在小鼠中研究HTR提供了高通量解决方案。
结论：利用磁耳标记和双相检测的HTR的高通量鉴定，采用侵入性较小的程序可提供令人满意的检测和辨别能力。
