Design of a degenerate primer pair to target a bacterial functional community: The hppd bacterial gene coding for the enzyme targeted by herbicides, a study case.

设计针对细菌功能群落的简并引物对 ： 编码除草剂靶向酶的 hppd 细菌基因 ，一个研究案例。

• 影响指数: 2.622
• 发表时间: 03 2020
• 来源期刊: J Microbiol Methods
• DOI: 10.1016/j.mimet.2020.105839
• PMID: 31926848
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 生物
• 作者列表: Thiour-Mauprivez C,Devers-Lamrani M,Mounier A,Beguet J,Spor A,Calvayrac C,Barthelmebs L,Martin-Laurent F

关键词: Degenerate primers; Hppd; Soil bacterial community

Mesh: 4-Hydroxyphenylpyruvate Dioxygenase / genetics; Actinobacteria / drug effects enzymology genetics; Bacteria / drug effects enzymology genetics; Cyanobacteria / drug effects enzymology genetics; Firmicutes / drug effects enzymology genetics; Genetic Variation / genetics; Herbicides / toxicity; Proteobacteria / drug effects enzymology genetics; Soil Microbiology

Abstract

The present work aimed to design a degenerate primer pair to target a large part of the hppd soil bacterial community, possibly affected by herbicides. We validated these primers by qPCR and high-throughput sequencing analysis of soil samples.

摘要

本工作旨在设计一种简并引物对，以靶向大部分hppd土壤细菌群落，可能受到除草剂的影响。我们通过qPCR和土壤样品的高通量测序分析验证了这些引物。