Abstract：
A reliable pigeon pea transformation system can assist the rapid improvement of this important grain legume through transgenic development. Here we describe two methods of Agrobacterium tumefaciens-mediated pigeon pea transformation. In the tissue culture based embryonic explant transformation method, microshoot grafting was included to obtain rapid root induction, while the other method was culture independent and designated as plumular meristem transformation. Both methods drastically enhanced the transformation frequency and have the potential to provide reasonable solutions for maximum transgenic recovery in biotechnological breeding programs.
摘要：
可靠的木豆转化系统可以通过转基因开发帮助这种重要的谷物豆科植物的快速改良。在这里，我们描述了根癌农杆菌介导的木豆转化的两种方法。在基于组织培养的胚胎外植体转化方法中，包括微芽嫁接以获得快速的根系诱导，而另一种方法是培养独立的，并被指定为羽毛分生组织转化。两种方法都大大提高了转化频率，并有可能为生物技术育种计划中的最大转基因回收提供合理的解决方案。
