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Abstract：
In a recent one-year feeding study, we observed no adverse effects on tissue level in organs of rats fed with the genetically-modified maize MON810. Here, we assessed RNA expression levels of 86 key genes of the apoptosis-, NF-кB-, DNA-damage response (DDR)-, and unfolded-protein response (UPR) pathways by RT-qPCR in the rat liver. Male and female rats were fed either with 33% MON810 (GMO), isogenic- (ISO), or conventional maize (CONV) and RNAs were quantified from eight rats from each of the six feeding groups. Only Birc2 transcript showed a significant (p ≤ 0.05) consistent difference of ≥1.5-fold between the GMO and ISO groups in both sexes. Unsupervised cluster analysis showed a strong separation of male and female rats, but no clustering of the feeding groups. Individual analysis of the pathways did not show any clustering of the male or female feeding groups either, though transcript levels of UPR pathway-associated genes caused some clustering of the male GMO and CONV feeding group samples. These differences were not seen between the GMO and ISO control or within the female cohort. Our data therefore does not support an adverse effect on rat liver RNA expression through the long-term feeding of MON810 compared to isogenic control maize.
摘要：
在最近的一项为期一年的喂养研究中，我们观察到转基因玉米MON810对大鼠器官组织水平的影响.这里,我们评估了凋亡的86个关键基因的RNA表达水平-，NF-kb-,DNA损伤反应（DDR）-，和通过RT-qPCR在大鼠肝脏中的未折叠蛋白反应（UPR）途径。给雄性和雌性大鼠喂食33%MON810(GMO),等基因-(ISO)，或常规玉米（CONV）和RNA定量来自六个喂养组的八只大鼠。只有Birc2转录物显示在两种性别的GMO和ISO组之间≥1.5倍的显着（p≤0.05）一致差异。无监督聚类分析显示雄性和雌性大鼠有很强的分离，但没有聚集的喂养组。对途径的个体分析也没有显示出男性或女性喂养组的任何聚类，尽管UPR途径相关基因的转录水平导致了雄性GMO和CONV喂养组样品的一些聚类。在GMO和ISO对照之间或在女性队列中没有看到这些差异。因此,与等基因对照玉米相比,我们的数据不支持通过长期饲喂MON810对大鼠肝脏RNA表达的不利影响。
