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Abstract：
Mosquitoes harbor an extensive diversity of \'insect-specific\' RNA viruses in addition to those important to human and animal health. However, because most studies of the mosquito virome have been conducted at lower latitudes, little is known about the diversity and evolutionary history of RNA viruses sampled from mosquitoes in northerly regions. Here, we compared the RNA virome of two common northern mosquito species, Culex pipiens and Culex torrentium, collected in south-central Sweden. Following bulk RNA-sequencing (meta-transcriptomics) of 12 libraries, comprising 120 specimens of Cx. pipiens and 150 specimens of Cx. torrentium, we identified 40 viruses (representing 14 virus families) of which 28 were novel based on phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) protein. Hence, we documented similar levels of virome diversity as in mosquitoes sampled from the more biodiverse lower latitudes. Many viruses were also related to those sampled on other continents, indicative of a widespread global movement and/or long host-virus co-evolution. Although the two mosquito species investigated have overlapping geographical distributions and share many viruses, several viruses were only found at a specific location at this scale of sampling, such that local habitat and geography may play an important role in shaping viral diversity in Culex mosquitoes.
摘要：
除对人类和动物健康重要的病毒外，蚊子还拥有广泛多样性的“昆虫特异性”RNA病毒。然而,因为大多数蚊子病毒学的研究都是在低纬度地区进行的，从北方地区的蚊子中取样的RNA病毒的多样性和进化史知之甚少。这里,我们比较了两种常见的北方蚊子的RNA病毒，淡色库蚊和铜色库蚊，收集在瑞典中南部。在对12个文库进行大量RNA测序（元转录组学）之后，由120个Cx标本组成。pipiens和Cx的150个标本。Torrenum,我们基于RNA依赖性RNA聚合酶(RdRp)蛋白的系统发育分析,鉴定出40种病毒(代表14个病毒家族),其中28种是新型病毒.因此，我们记录了与从生物多样性较高的低纬度地区采样的蚊子相似的病毒多样性水平。许多病毒也与其他大陆的病毒有关，表明广泛的全球运动和/或长期宿主病毒共同进化。尽管调查的两种蚊子具有重叠的地理分布并共享许多病毒，在这种规模的采样中，仅在特定位置发现了几种病毒，因此，当地的栖息地和地理可能在塑造库蚊的病毒多样性方面发挥重要作用。
