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Abstract：
Glucagon circulates in concentrations in the low picomolar range, which is demanding regarding the sensitivity of the methods for quantification applied. In addition, the differential and tissue specific proteolytic processing of the glucagon precursor and the presence in of several glucagon-like sequences, not only in the precursor of glucagon, but also in a number of other peptides of the glucagon-secretin family of peptides, put special demands on the specificity of the assays. Finally, experience has shown that unspecific interference of plasma components has presented additional problems. All of these problems have resulted in a lot of diverging results concerning measured and reported glucagon responses in both humans and experimental animals that have and still are causing considerable debate and controversy. There is very solid evidence that glucagon is an important hormone in human and mammalian metabolism, but its precise physiological role in glucose and lipid metabolism and in metabolic disease has been difficult to establish, not least because of these difficulties. It was our purpose with this review to discuss the methods of glucagon quantification and discuss pitfalls and sources of error. We also reviewed some of the dogmas regarding glucagon secretion in the light of the methodological difficulties.
摘要：
胰高血糖素以低皮摩尔范围的浓度循环，这对应用的定量方法的灵敏度要求很高。此外,胰高血糖素前体的差异和组织特异性蛋白水解过程以及几种胰高血糖素样序列的存在，不仅在胰高血糖素的前体中，而且在胰高血糖素-促胰液素肽家族的许多其他肽中，对测定的特异性提出特殊要求。最后,经验表明，等离子体成分的非特定干扰带来了额外的问题。所有这些问题都导致了许多关于人类和实验动物中测量和报道的胰高血糖素反应的不同结果，这些结果已经并且仍然引起相当大的争论和争议。有非常确凿的证据表明胰高血糖素是人类和哺乳动物代谢中的重要激素，但其在糖脂代谢和代谢性疾病中的精确生理作用一直难以确立,尤其是因为这些困难。我们的目的是讨论胰高血糖素定量的方法，并讨论陷阱和错误来源。鉴于方法学上的困难，我们还回顾了有关胰高血糖素分泌的一些教条。
