PDF(Pubmed)
Abstract：
Legionella species are widespread in natural water sources and man-made aqueous environments, as well as fresh-water. The present study was conducted owing to the lack of research regarding the prevalence of Legionella spp in the water sources of Ahvaz city in southwest Iran. In this study the macrophage infectivity potentiator (mip) gene sequencing was used for identification of various Legionella species isolated from different water sources. In this study, 144 water samples were collected and inoculated on the buffered charcoal-yeast extract (BCYE) agar and modified Wadowsky-Yee (MWY) medium. The DNA was extracted from positive cultures. The Legionella species were confirmed by amplifying a 654 bp fragment of the 16S rRNA gene. The mip gene of all isolates were amplified by PCR and purified for sequencing. The mip gene sequences were analyzed by jPHYDIT software version 1. The results showed a 13.9% (20/144) prevalence of Legionella spp. in water sources of Ahvaz city, southwest Iran. Analyzing of the mip gene sequences showed, out of 20 Legionella isolates, 13 isolates (54.1%) were positive for L. pneumophila, 5 isolates (20.8%) were positive for L. worsleinsis, one isolates for each one of L. dumoffi and L. fairfieldensis, (4.1%). According to our research, the occurrence of Legionella spp in water sources could be a hazard for the health systems especially in the hospitals. The regular monitoring of these water sources by health planners may therefore be useful for decreasing the risk for Legionella spp. infections.
摘要：
军团菌种类广泛存在于天然水源和人造水环境中,以及淡水。本研究是由于缺乏有关伊朗西南部阿瓦士市水源中军团菌属流行的研究而进行的。在这项研究中，巨噬细胞感染性增效剂（mip）基因测序用于鉴定从不同水源分离的各种军团菌。在这项研究中,收集144个水样并接种在缓冲木炭-酵母提取物(BCYE)琼脂和改良的Wadowsky-Yee(MWY)培养基上。从阳性培养物中提取DNA。通过扩增16SrRNA基因的654bp片段来确认军团菌物种。通过PCR扩增所有分离物的mip基因并纯化用于测序。通过jPHYDIT软件版本1分析mip基因序列。结果显示军团菌的患病率为13.9%(20/144)。在阿瓦士市的水源，伊朗西南部。对mip基因序列的分析显示，在20个军团菌分离株中，13株(54.1%)肺炎杆菌阳性,5个分离株(20.8%)对苦力乳杆菌呈阳性,DumoffiL.和FairfieldensisL.(4.1%)。根据我们的研究,水源中军团菌的发生可能对卫生系统构成危害，尤其是在医院中。因此，健康计划人员对这些水源进行定期监测可能有助于降低军团菌的风险。感染。
