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Abstract：
Purpose/Aim: Skeletal muscle tissue explants have been cultured and studied for nearly 100 years. These cultures, which retain complex tissue structure in an environment suited to precision manipulation and measurement, have led to seminal discoveries of the extrinsic and intrinsic mechanisms regulating contractility, metabolism and regeneration. This review discusses the two primary models of muscle explant: isolated myofiber and intact muscle.Materials and Methods: Relevant literature was reviewed and synthesized with a focus on the unique challenges and capabilities of each explant model.Results: Impactful past, current and future novel applications are discussed.Conclusions: Experiments using skeletal muscle explants have been integral to our understanding of the fundamentals of muscle physiology. As they are refined and adapted, they are poised to continue to inform the field for years to come.
摘要：
目的：骨骼肌组织外植体已经培养和研究了近100年。这些文化,在适合精确操作和测量的环境中保留复杂的组织结构，已经导致了调节收缩性的外在和内在机制的开创性发现，新陈代谢和再生。本文讨论了肌肉外植体的两种主要模型：分离的肌纤维和完整的肌肉。材料和方法：回顾和综合了相关文献，重点是每个外植体模型的独特挑战和能力。结果：影响过去，讨论了当前和未来的新应用。结论：使用骨骼肌外植体的实验对于我们对肌肉生理学基础的理解是不可或缺的。当它们被提炼和适应时，他们准备在未来几年继续为该领域提供信息。
