PDF(Sci-hub)
Abstract：
Dental unit waterlines (DUWLs) can be considered one of the possible routes of H. pylori transmission, although its presence in DUWLs has not yet been investigated thoroughly. The present study aimed to discover the prevalence of H. pylori and oral streptococci (S. oralis and S. mutans) in DUWLs to evaluate the risk of exposure to human pathogens in dental practices. We collected the output water from 60 dental chair units (DCUs) in 26 private dentistry settings in Turin, searching for H. pylori and oral streptococci (OS) DNA, with a polymerase chain reaction (PCR) technique. At the same time, dentists completed a questionnaire about their DCUs, their main activities, the presence of anti-retraction devices, their attitudes about disinfection, etc. No dental chair unit tested was contaminated with H. pylori or S. mutans; only one dental chair was contaminated with S. oralis (1.7%). Considering the results, we can state that: (i) the lack of H. pylori DNA in water samples analyzed, suggests that municipal water is presumably treated with a sufficient chlorine level to inactivate DNA over time; (ii) the aspiration of oral fluids is limited by anti-retraction valves fitted distally to hand pieces; (iii) propidium monoazide qPCR (PMA-qPCR) could be a good technique to investigate and monitor potential environmental sources of infections such as DUWLs.
摘要：
牙科单位水线（DUWL）可以被认为是幽门螺杆菌传播的可能途径之一，尽管它在DUWL中的存在尚未得到彻底调查。本研究旨在发现幽门螺杆菌和口腔链球菌的患病率(S.口腔和变形链球菌）在DUWL中评估牙科实践中暴露于人类病原体的风险。我们从都灵26个私人牙科环境中的60个牙科椅单元（DCU）中收集了输出水，寻找幽门螺杆菌和口腔链球菌(OS)DNA，使用聚合酶链反应（PCR）技术。同时,牙医完成了关于他们的DCU的调查问卷，其主要活动,防缩装置的存在，他们对消毒的态度，等。测试的牙科椅单元没有被幽门螺杆菌或变形链球菌污染;只有一把牙科椅被口腔链球菌污染(1.7%)。考虑到结果，我们可以说：(I)分析的水样中缺乏幽门螺杆菌DNA，表明市政用水可能经过足够的氯水平处理，以使DNA随时间失活；（ii）口腔液的抽吸受到远端安装在手持件上的防回缩阀的限制；（iii）单叠氮化物丙啶qPCR（PMA-qPCR）可能是调查和监测DUWL等潜在感染环境源的良好技术。
