METHODS: Our study included 24 adult T1DM patients diagnosed with peripheral vascular complications. The patients were exposed to 10 sessions of 100% oxygen inhalation at 2.4 atmosphere absolute for 1 hour. Blood samples were collected at admission and after HBOT for measurement of metabolic parameters, FA composition and IGFBP-1. Measurement of plasma FA composition was determined by gas chromatography. Expression of IGFBP-1 in the serum was estimated by Western blot analysis.
RESULTS: HBOT decreased blood levels of total cholesterol (p<0.05), triglycerides (p<0.05) and low-density lipoprotein (p<0.05). HBOT increased plasma levels of individual FAs: palmitic acid (p<0.05), palmitoleic acid (p<0.05), docosapentaenoic acid (p<0.05) and docosahexaenoic acid (p<0.01), and decreased levels of stearic acid (p<0.05), alpha linolenic acid (p<0.05) and linoleic acid (p<0.01). Expression of IGFBP-1 (p<0.01) was increased, whereas the level of insulin (p<0.001) was decreased in the serum after HBOT.
CONCLUSIONS: Our results indicate that HBOT exerts beneficial effects in T1DM patients by improving the lipid profile and altering FA composition.
方法:我们的研究包括24名诊断为外周血管并发症的成人T1DM患者。患者在2.4绝对大气压下暴露于10次100%氧气吸入1小时。入院时和HBOT后收集血液样本用于测量代谢参数,FA组成和IGFBP-1。血浆FA组成的测量通过气相色谱法测定。通过Western印迹分析估计血清中IGFBP-1的表达。
结果:HBOT降低了血液中总胆固醇水平(p<0.05),甘油三酯(p<0.05)和低密度脂蛋白(p<0.05)。HBOT增加了单个FAs:棕榈酸的血浆水平(p<0.05),棕榈油酸(p<0.05),二十二碳五烯酸(p<0.05)和二十二碳六烯酸(p<0.01),硬脂酸含量降低(p<0.05),α亚麻酸(p<0.05)和亚油酸(p<0.01)。IGFBP-1的表达增加(p<0.01),而HBOT后血清胰岛素水平(p<0.001)下降。
结论:我们的结果表明,HBOT通过改善血脂和改变FA组成在T1DM患者中发挥有益作用。