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Abstract：
Tea plant is an important economic crop on a global scale. Its yield and quality are affected by abiotic stress. The calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) family genes play irreplaceable roles in plant development and stress resistance. More and more CBL-CIPK genes have been identified, but a few CBL-CIPK genes have been cloned and characterized in tea plants. In this study, 7 CsCBLs and 18 CsCIPKs were identified based on the tea plant genome. Physicochemical properties, phylogenetic, conserved motifs, gene structure, homologous gene network, and promoter upstream elements of these 25 genes were analyzed. Conserved motifs of these genes varied with phylogenetic tree node. From the genetic structure, members of the tea plant CIPK gene family can be divided into two types: intron rich and no intron. Many stress-related elements were found in the 2000 bp upstream of the promoter, and PlantCARE predicted that CsCBL4 contained 30 stress-related elements. PlantPAN2 shows that CsCIPK6 contains 48 ABRELATERD1; CsCIPK17 contains 37 GT1CONSENSUS; CsCIPK3 contains 64 MYBCOREATCYCB1; CsCBL3 contains 52 SORLIP1AT; CsCBL5 contains 65 SURECOREATSULTR11; and CsCIPK11 contains 83 WBOXATNPR1. In addition, eight genes were selected for quantitative real-time PCR (RT-qPCR) to detect their expression profiles under high-temperature, low-temperature, salt, and drought treatments. These genes were found to be responsive to one or more abiotic stress treatments. The expression levels of CsCBL4, CsCIPK2, and CsCIPK14 were similar, and they were homologous to AtSOS3 and AtSIP3 and AtSIP4 in Arabidopsis, which were involved in the SOS pathway. This study provides insight into the potential functions of the CsCBL and CsCIPK of tea plant.
摘要：
茶树是全球规模内重要的经济作物。其产量和品质受到非生物胁迫的影响。钙调神经磷酸酶B样蛋白(CBL)和CBL相互作用蛋白激酶(CIPK)家族基因在植物发育和抗逆性中起着不可替代的作用。越来越多的CBL-CIPK基因被鉴定,但是一些CBL-CIPK基因已经在茶树中被克隆和表征。在这项研究中,基于茶树基因组鉴定了7个CsCBLs和18个CsCIPKs。物理化学性质,系统发育，保守的图案，基因结构，同源基因网络，分析了这25个基因的启动子上游元件。这些基因的保守基序随系统发育树节点而变化。从遗传结构来看，茶树CI一PK基因家族的成员可分为两类：富含内含子和无内含子。在启动子上游的2000bp中发现了许多与胁迫相关的元件，和PlantCARE预测CsCBL4包含30个与应激相关的元素。PlantPAN2显示CsCIPK6包含48个ABRELATERD1；CsCIPK17包含37个GT1CONSENSUS；CsCIPK3包含64个MYBCOREATCYCB1；CsCBL3包含52个SORLIP1AT；CsCBL5包含65个SURECOATSULT11；CIPK11包含83WBOXATNPR1此外,选择8个基因进行实时定量PCR(RT-qPCR)检测其在高温下的表达谱,低温,盐,和干旱处理。发现这些基因对一种或多种非生物胁迫处理有反应。CsCBL4、CsCIPK2和CsCIPK14的表达水平相似，它们与拟南芥中的AtSOS3和AtSIP3和AtSIP4同源，参与了SOS途径。本研究为茶树CsCBL和CsCIPK的潜在功能提供了见解。
