Mesh : Animals Biomarkers, Tumor / genetics CRISPR-Cas Systems / genetics Carcinoma, Non-Small-Cell Lung / diagnosis genetics therapy Cell Line, Tumor Chromosomes, Human, Pair 3 / genetics Chromosomes, Human, Pair 5 / genetics Chromosomes, Human, Pair 9 / genetics Clinical Trials as Topic Gene Editing / methods Gene Knockout Techniques Genetic Therapy / methods Humans Lung Neoplasms / diagnosis genetics therapy Mice Mutation Sequence Deletion Translational Research, Biomedical / methods Tumor Suppressor Proteins / genetics

来  源:   DOI:10.1038/s41417-019-0116-8   PDF(Sci-hub)

Abstract:
Gene therapy using CRISPR Cas9 technique is rapidly gaining popularity among the scientific community primarily because of its versatility, cost-effectiveness, and high efficacy. While the laboratory-based experiments and findings making use of CRISPR as a gene editing tool are available in ample amounts, the question arises that how much of these findings are actually translatable into measures helping in combating particular disease conditions. In this review, we highlight the important studies and findings done till now in the perspective of lung cancer with an in-depth analysis of various clinical trials associated with the use of CRISPR Cas9 technology in the field of cancer research.
摘要:
使用CRISPRCas9技术的基因治疗在科学界迅速普及,主要是因为它的多功能性,成本效益,和高疗效。虽然利用CRISPR作为基因编辑工具的实验室实验和发现有大量可用,问题是,这些发现中有多少实际上可以转化为有助于对抗特定疾病的措施。在这次审查中,我们通过深入分析与CRISPRCas9技术在癌症研究领域的使用相关的各种临床试验,重点介绍了迄今为止在肺癌领域所做的重要研究和发现.
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