关键词: Antioxidant enzymes Elevated ozone concentration PAGE Reactive oxygen species

Mesh : Antioxidants / metabolism Ascorbate Peroxidases / metabolism Catalase / metabolism Oryza / drug effects enzymology genetics growth & development Oxidation-Reduction / drug effects Ozone / toxicity Peroxidase / metabolism Reactive Oxygen Species / metabolism Stress, Physiological Superoxide Dismutase / metabolism

来  源:   DOI:10.1007/s00128-019-02653-7   PDF(Sci-hub)

Abstract:
Free-air O3 enrichment was used to investigate the responses of different antioxidant mechanisms in different rice (Oryza sativa L.) cultivars - O3-sensitive hybrid indica (O3-S) cultivars and O3-tolerant conventional japonica (O3-T) cultivars across all growth stages. Elevated [O3] induced increases in reactive oxygen species (ROS) production in O3-S cultivars, which were more pronounced in the later growing stages. In O3-S cultivars, continuous O3 stress decreased catalase (CAT), peroxidase (POD) and glutathione peroxidase (GPX) activities, while in O3-T cultivars, short-term O3 stress decreased superoxide dismutase (SOD), CAT, POD and GPX activities. The same POD isozyme patterns were observed in both O3-S and O3-T cultivars, while SOD and APX isozymes varied by cultivar. The results suggest that O3 tolerance might be improved at different rice development stages through regulating the responses of antioxidant mechanisms to O3 stress.
摘要:
自由空气O3富集用于研究不同水稻(OryzasativaL.)品种-O3敏感的杂种in(O3-S)品种和耐O3的常规粳稻(O3-T)品种中不同抗氧化机制的响应。所有生长阶段。升高的[O3]诱导了O3-S品种中活性氧(ROS)产生的增加,这在后期的生长阶段更为明显。在O3-S品种中,连续O3胁迫降低过氧化氢酶(CAT),过氧化物酶(POD)和谷胱甘肽过氧化物酶(GPX)活性,而在O3-T品种中,短期O3胁迫降低超氧化物歧化酶(SOD),CAT,POD和GPX活动。在O3-S和O3-T品种中观察到相同的POD同工酶模式,而SOD和APX同工酶因品种而异。结果表明,通过调节抗氧化机制对O3胁迫的响应,可能会在水稻不同发育阶段提高对O3的耐受性。
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