Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris.

• 发表时间: 2019
• 来源期刊: Methods Mol Biol
• DOI: 10.1007/978-1-4939-9024-5_14
• PMID: 30737747
• 文章类型: Journal Article
• 作者列表: Zahid M,Rinas U

关键词: HBsAg; Pichia pastoris; Vaccine; Virus; Virus-like particle

Mesh: Biotechnology / methods; Hepatitis B Surface Antigens / genetics metabolism; Pichia / genetics metabolism

Abstract

Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.