Antisense RNA-Based Strategy for Enhancing Surfactin Production in Bacillus subtilis TS1726 via Overexpression of the Unconventional Biotin Carboxylase II To Enhance ACCase Activity.

• 影响指数: 5.249
• 发表时间: 02 2019 15
• 来源期刊: ACS Synth Biol
• DOI: 10.1021/acssynbio.8b00459
• PMID: 30702274
• 文章类型: Journal Article
• 中科院分区: Q2
• 方向: 生物
• 作者列表: Wang M,Yu H,Shen Z

关键词: Bacillus subtilis; acetyl-CoA carboxylase; biotin carboxylase; surfactin

Mesh: Acetyl-CoA Carboxylase / genetics metabolism; Bacillus subtilis / genetics; Biotin / genetics metabolism; Carbon-Nitrogen Ligases / genetics metabolism; RNA, Antisense / genetics

Abstract

The antisense RNA (asRNA) strategy is commonly used to block protein expression and downregulate the contents of metabolites in several microorganisms. Here, we show that the asRNA strategy can also be used to block gfp expression in Bacillus subtilis TS1726, which could further be utilized in the identification of new genes and functions. Via application of this strategy, biotin carboxylase II encoded by yngH (GeneID 939474) was identified to play a more significant role in maintaining acetyl-CoA carboxylase (ACCase) activity and enhancing surfactin synthesis compared to those of other ACCase subunits. The yngH gene was then overexpressed in the engineered strain B. subtilis TS1726(yngH). The surfactin titer of TS1726(yngH) increased to 13.37 g/L in a flask culture, representing a 43% increase compared to that of parental strain TS1726. This strategy opens the door to achieving large-scale production and broad application of surfactin.