Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.

DNA 重组和修复研究指南 ： DNA 修复途径的细胞测定。

• 影响指数: 5.316
• 发表时间: Jan 2019 7
• 来源期刊: Microb Cell
• DOI: 10.15698/mic2019.01.664
• PMID: 30652105
• 文章类型: Journal Article
• 作者列表: Klein HL,Bačinskaja G,Che J,Cheblal A,Elango R,Epshtein A,Fitzgerald DM,Gómez-González B,Khan SR,Kumar S,Leland BA,Marie L,Mei Q,Miné-Hattab J,Piotrowska A,Polleys EJ,Putnam CD,Radchenko EA,Saada AA,Sakofsky CJ,Shim EY,Stracy M,Xia J,Yan Z,Yin Y,Aguilera A,Argueso JL,Freudenreich CH,Gasser SM,Gordenin DA,Haber JE,Ira G,Jinks-Robertson S,King MC,Kolodner RD,Kuzminov A,Lambert SA,Lee SE,Miller KM,Mirkin SM,Petes TD,Rosenberg SM,Rothstein R,Symington LS,Zawadzki P,Kim N,Lisby M,Malkova A

关键词: DNA breaks; DNA repair centers; DNA resection; DSBs; Holliday junctions; R-loops; chromatin dynamics; chromosome rearrangements; crossovers; fluorescent proteins; gene amplification; gene conversion; genome instability; gross chromosome rearrangements; homologous recombination; mitotic recombination; mutagenesis; pulsed field gel electrophoresis; replication fork stalling; single-particle tracking; sister chromatid recombination; sister repetitive sequences; site-specific chromosome breaks; toxic recombination intermediates; yeast artificial chromosome

Abstract

Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

摘要

重组试验极大地帮助了理解基因组的可塑性，修复和诱变。这些测定已经在微生物系统中开发,其提供了可以容易地操作的遗传和分子报道分子的优点。细胞测定包括遗传，分子，和细胞学记者。分析是强大的工具，但每一个都有其特定的优点和局限性。这里回顾了最常用的检测方法，讨论,并作为未来研究的指导方针提出。