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Abstract：
Interleukin 15 (IL15) is a pleiotropic cytokine that participates in innate and adaptive immunity along with its receptor α-chain (IL15Rα). In order to investigate the potential roles of IL15 and IL15Rα in dojo loach (Misgurnus anguillicaudatus), we firstly cloned the cDNA sequence of Ma-IL15 and Ma-IL15Rα, which contain 1096bp and 1236bp and code proteins of 193 amino acids and 210 amino acids, respectively. A short signal peptide and Pfam IL15 domain were found in Ma-IL15, while a highly conserved sushi domain existed in Ma-IL15Rα. Ontogeny analysis indicated that significantly increased expression of Ma-IL15 and Ma- IL15Rα mRNA were detected in larvae from 1d to 7d post hatching, while relative high expression levels were detected in both systematic and mucosal immune-related tissues of adult dojo loach. Then three dojo loach infection models with F. columnare G4, I. multifiliis and Saprolegnia parasitica were constructed, which resulted in increased skin goblet cells and serious lesions in gill. Ma-IL15 and Ma-IL15Rα showed different expression patterns in different tissues during three infection models. Ma-IL15Rα mRNA was found to be more significantly elevated than Ma-IL15 after infection with F. columnare G4 in all examined tissues including kidney, spleen, gill and skin. I. multifiliis infection induced higher expression of Ma-IL15 in mucosal tissues including skin and gill, while it mainly increased Ma-IL15Rα expression in kidney. Moreover, our study firstly evaluated the influence of fungal infection on IL15 and IL15Rα expression in teleost, and it is interesting to find that both Ma-IL15 and Ma-IL15Rα expression showed consistent up-regulation after Saprolegnia parasitica infection compared to two other infection models. Therefore, our results suggest that Ma-IL15 and Ma-IL15Rα possess important defensive roles in systematic and mucosal tissues of dojo loach during bacterial, fungal and parasitic infection.
摘要：
白细胞介素15（IL15）是一种多效性细胞因子，与其受体α链（IL15Rα）一起参与先天和适应性免疫。为了研究IL15和IL15Rα在dojo泥潭（Misgurnusanguillicaudatus）中的潜在作用，我们首先克隆了Ma-IL15和Ma-IL15Rα的cDNA序列，包含1096bp和1236bp，编码193个氨基酸和210个氨基酸的蛋白质，分别。在Ma-IL15中发现了一个短信号肽和PfamIL15结构域，而在Ma-IL15Rα中存在一个高度保守的寿司结构域。个体发育分析表明，孵化后1d至7d，幼虫中Ma-IL15和Ma-IL15RαmRNA的表达显着增加。而在成年dojo泥舟的系统和粘膜免疫相关组织中均检测到相对较高的表达水平。然后构建了三个带有F.columnareG4，I.multifiliis和寄生腐殖质的dojo泥法菌感染模型，导致皮肤杯状细胞增加和g的严重损伤。在三种感染模型中，Ma-IL15和Ma-IL15Rα在不同组织中表现出不同的表达模式。发现Ma-IL15RαmRNA在感染F.columnareG4后比Ma-IL15显著升高，在所有检查的组织中，包括肾脏，脾,脾ill和皮肤。I.Multifiliis感染诱导Ma-IL15在包括皮肤和g在内的粘膜组织中的较高表达，而主要增加肾脏Ma-IL15Rα的表达。此外，我们的研究首先评估了真菌感染对硬骨鱼IL15和IL15Rα表达的影响，有趣的是，与其他两种感染模型相比，在寄生虫感染后，Ma-IL15和Ma-IL15Rα表达均显示一致的上调。因此,我们的结果表明，在细菌期间，Ma-IL15和Ma-IL15Rα在dojo泥的系统和粘膜组织中具有重要的防御作用，真菌和寄生虫感染。
