PDF(Pubmed)
Abstract：
Translational correlates to pain with activities after deep tissue injury have been rarely studied. We hypothesized that deep tissue incision causes greater activation of nociception-transmitting neurons evoked by muscle contraction. In vivo neuronal activity was recorded in 203 dorsal horn neurons (DHNs) from 97 rats after sham, skin-only, or skin + deep muscle incision. We evaluated DHN responses to static, isometric muscle contractions induced by direct electrical stimulation of the muscle. The effect of pancuronium on DHN response to contractions was also examined. Approximately 50% of DHNs with receptive fields in the hindpaw were excited during muscle contraction. One-second .5- and 1.0-g muscle contractions produced greater DHN activity after skin + deep muscle incision (median [interquartile range], 32 [5-39] impulses, P = .021; and 36 [26-46] impulses, P = .006, respectively) than after sham (6 [0-21] and 15 [8-32] impulses, respectively). Neuromuscular blockade with pancuronium inhibited the muscle contractions and DHN activation during electrical stimulation, demonstrating contraction-induced activation. The greater response of spinal DHNs to static muscle contraction after skin + deep muscle incision may model and inform mechanisms of dynamic pain after surgery. PERSPECTIVE: Completion of various activities is an important milestone for recovery and hospital discharge after surgery. Skin + deep muscle incision caused greater activation of nociception-transmitting DHNs evoked by muscle contraction compared with skin-only incision. This result suggests an important contribution of deep muscle injury to activity-evoked hyperalgesia after surgery.
摘要：
很少研究深层组织损伤后疼痛与活动的转化相关性。我们假设深层组织切口会导致肌肉收缩引起的伤害性感受传递神经元的更大激活。假手术后，在97只大鼠的203只背角神经元（DHN）中记录了体内神经元活性，仅限皮肤,或皮肤+深层肌肉切口。我们评估了DHN对静态，直接电刺激肌肉引起的等距肌肉收缩。还检查了潘库溴铵对DHN对收缩反应的影响。在肌肉收缩过程中，大约有50％的后爪有感受野的DHN被兴奋。1秒0.5-和1.0-g肌肉收缩在皮肤+深肌肉切口后产生更大的DHN活性（中位数[四分位间距]，32[5-39]冲动，P=.021;和36[26-46]脉冲，P=.006，分别）比假手术后（6[0-21]和15[8-32]冲动，分别)。神经肌肉阻滞与潘库溴铵抑制肌肉收缩和DHN激活在电刺激，显示收缩诱导的激活。脊柱DHN对皮肤+深肌切口后的静态肌肉收缩的更大反应可以模拟和告知手术后动态疼痛的机制。前景：完成各种活动是手术后康复和出院的重要里程碑。与仅皮肤切口相比，皮肤深层肌肉切口引起的肌肉收缩引起的伤害性传递DHN的激活更大。该结果表明，深部肌肉损伤对手术后活动引起的痛觉过敏有重要贡献。
