Abstract：
Due to recent advances in experimental and theoretical approaches, the dynamic three-dimensional organization (3D) of the nucleus has become a very active area of research in life sciences. We now understand that the linear genome is folded in ways that may modulate how genes are expressed during the basic functioning of cells. Importantly, it is now possible to build 3D models of how the genome folds within the nucleus and changes over time (4D). Because genome folding influences its function, this opens exciting new possibilities to broaden our understanding of the mechanisms that determine cell fate. However, the rapid evolution of methods and the increasing complexity of data can result in ambiguity and reproducibility challenges, which may hamper the progress of this field. Here, we describe such challenges ahead and provide guidelines to think about strategies for shared standardized validation of experimental 4D nucleome data sets and models.
摘要：
由于实验和理论方法的最新进展，核的动态三维组织（3D）已成为生命科学研究中非常活跃的领域。我们现在了解到，线性基因组的折叠方式可能会调节基因在细胞基本功能过程中的表达方式。重要的是,现在可以建立基因组如何在细胞核内折叠并随时间变化的3D模型（4D）。因为基因组折叠会影响它的功能，这开启了令人兴奋的新可能性，以扩大我们对决定细胞命运的机制的理解。然而,方法的快速发展和数据复杂性的增加可能导致歧义和可重复性的挑战，这可能会阻碍这一领域的发展。这里,我们描述了这些未来的挑战,并提供了思考实验4D核体数据集和模型的共享标准化验证策略的指南.
