Genome-wide association study identifies multiple new loci associated with Ewing sarcoma susceptibility.

• 影响指数: 17.694
• 发表时间: 08 2018 9
• 来源期刊: Nat Commun
• DOI: 10.1038/s41467-018-05537-2
• PMID: 30093639
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 综合性期刊
• 作者列表: Machiela MJ,Grünewald TGP,Surdez D,Reynaud S,Mirabeau O,Karlins E,Rubio RA,Zaidi S,Grossetete-Lalami S,Ballet S,Lapouble E,Laurence V,Michon J,Pierron G,Kovar H,Gaspar N,Kontny U,González-Neira A,Picci P,Alonso J,Patino-Garcia A,Corradini N,Bérard PM,Freedman ND,Rothman N,Dagnall CL,Burdett L,Jones K,Manning M,Wyatt K,Zhou W,Yeager M,Cox DG,Hoover RN,Khan J,Armstrong GT,Leisenring WM,Bhatia S,Robison LL,Kulozik AE,Kriebel J,Meitinger T,Metzler M,Hartmann W,Strauch K,Kirchner T,Dirksen U,Morton LM,Mirabello L,Tucker MA,Tirode F,Chanock SJ,Delattre O

Mesh: Alleles; Cell Cycle Proteins / genetics; Cell Proliferation / genetics; DNA-Binding Proteins / genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genetic Predisposition to Disease; Genome-Wide Association Study; Genotype; Homeobox Protein Nkx-2.2; Homeodomain Proteins / genetics; Humans; Nuclear Proteins; Oncogene Proteins, Fusion / genetics; Polymorphism, Single Nucleotide; Proto-Oncogene Protein c-fli-1 / genetics; Quality Control; Quantitative Trait Loci; RNA-Binding Protein EWS / genetics; Risk; Sarcoma, Ewing / ethnology genetics; Transcription Factors / genetics; Whites; Zebrafish Proteins

Abstract

Ewing sarcoma (EWS) is a pediatric cancer characterized by the EWSR1-FLI1 fusion. We performed a genome-wide association study of 733 EWS cases and 1346 unaffected individuals of European ancestry. Our study replicates previously reported susceptibility loci at 1p36.22, 10q21.3 and 15q15.1, and identifies new loci at 6p25.1, 20p11.22 and 20p11.23. Effect estimates exhibit odds ratios in excess of 1.7, which is high for cancer GWAS, and striking in light of the rarity of EWS cases in familial cancer syndromes. Expression quantitative trait locus (eQTL) analyses identify candidate genes at 6p25.1 (RREB1) and 20p11.23 (KIZ). The 20p11.22 locus is near NKX2-2, a highly overexpressed gene in EWS. Interestingly, most loci reside near GGAA repeat sequences and may disrupt binding of the EWSR1-FLI1 fusion protein. The high locus to case discovery ratio from 733 EWS cases suggests a genetic architecture in which moderate risk SNPs constitute a significant fraction of risk.