PDF(Pubmed)
Abstract：
BACKGROUND: Flax is an important field crop that can be used for either oilseed or fiber production. Plant height and technical length are important characters for flax. For linseed flax, plants usually have a short technical length and plant height than those for fiber flax. As an important agronomical character for fiber and linseed flax, plant height is usually a selection target for breeding. However, because of limited technologies and methods available, there has been little research focused on discovering the molecular mechanism controlling plant height.
RESULTS: In this study, two related recombinant inbred line (RIL) populations developed from crosses of linseed and fiber parents were developed and phenotyped for plant height and technical length in four environments. A consensus linkage map based on two RIL populations was constructed using SNP markers generated by genotyping by sequencing (GBS) technology. A total of 4497 single nucleotide polymorphism (SNP) markers were included on 15 linkage groups with an average marker density of one marker every 2.71 cM. Quantitative trait locus (QTL) mapping analysis was performed for plant height and technical length using the two populations. A total of 19 QTLs were identified for plant height and technical length. For the MH population, eight plant height QTLs and seven technical length QTLs were identified, five of which were common QTLs for both traits. For the PH population, six plant height and three technical length QTLs were identified. By comparing the QTLs and candidate gene information in the two population, two common QTLs and three candidate genes were discovered.
CONCLUSIONS: This study provides a foundation for map-based cloning of QTLs and marker-assisted selection for plant height-related traits in linseed and fiber flax.
RESULTS: In this study, two related recombinant inbred line (RIL) populations developed from crosses of linseed and fiber parents were developed and phenotyped for plant height and technical length in four environments. A consensus linkage map based on two RIL populations was constructed using SNP markers generated by genotyping by sequencing (GBS) technology. A total of 4497 single nucleotide polymorphism (SNP) markers were included on 15 linkage groups with an average marker density of one marker every 2.71 cM. Quantitative trait locus (QTL) mapping analysis was performed for plant height and technical length using the two populations. A total of 19 QTLs were identified for plant height and technical length. For the MH population, eight plant height QTLs and seven technical length QTLs were identified, five of which were common QTLs for both traits. For the PH population, six plant height and three technical length QTLs were identified. By comparing the QTLs and candidate gene information in the two population, two common QTLs and three candidate genes were discovered.
CONCLUSIONS: This study provides a foundation for map-based cloning of QTLs and marker-assisted selection for plant height-related traits in linseed and fiber flax.
摘要：
背景：亚麻是一种重要的大田作物，可用于油料种子或纤维生产。植物高度和技术长度是亚麻的重要特征。对于亚麻亚麻，植物通常比纤维亚麻的技术长度和植物高度短。作为纤维和亚麻亚麻的重要农艺性状，株高通常是育种的选择目标。然而,由于可用的技术和方法有限，很少有研究集中在发现控制植物高度的分子机制。
结果：在这项研究中，从亚麻籽和纤维亲本的杂交中开发了两个相关的重组自交系（RIL）种群，并在四种环境中对株高和技术长度进行了表型分析。使用通过测序（GBS）技术进行基因分型产生的SNP标记，构建了基于两个RIL群体的共有连锁图谱。在15个连锁组中包含总共4497个单核苷酸多态性（SNP）标记，平均标记密度为每2.71cM一个标记。使用两个种群对植物高度和技术长度进行了数量性状基因座（QTL）定位分析。总共确定了19个QTL的植物高度和技术长度。对于MH人口来说，确定了八个株高QTL和七个技术长度QTL，其中五个是两个性状的共同QTL。对于PH人群，确定了6株高和3个技术长度QTL。通过比较两个群体的QTL和候选基因信息,发现了两个常见的QTL和三个候选基因。
结论：本研究为亚麻籽和纤维亚麻的QTL图谱克隆和植物高度相关性状的标记辅助选择奠定了基础。
结果：在这项研究中，从亚麻籽和纤维亲本的杂交中开发了两个相关的重组自交系（RIL）种群，并在四种环境中对株高和技术长度进行了表型分析。使用通过测序（GBS）技术进行基因分型产生的SNP标记，构建了基于两个RIL群体的共有连锁图谱。在15个连锁组中包含总共4497个单核苷酸多态性（SNP）标记，平均标记密度为每2.71cM一个标记。使用两个种群对植物高度和技术长度进行了数量性状基因座（QTL）定位分析。总共确定了19个QTL的植物高度和技术长度。对于MH人口来说，确定了八个株高QTL和七个技术长度QTL，其中五个是两个性状的共同QTL。对于PH人群，确定了6株高和3个技术长度QTL。通过比较两个群体的QTL和候选基因信息,发现了两个常见的QTL和三个候选基因。
结论：本研究为亚麻籽和纤维亚麻的QTL图谱克隆和植物高度相关性状的标记辅助选择奠定了基础。
