Abstract：
Diseases caused by livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) are an important global public health concern, and MRSA is increasingly being isolated in bovine milk. However, information on the genotype and antimicrobial resistance of MRSA in bovine milk in Xinjiang is limited. The objective of this study was to determine the antimicrobial-susceptible phenotypes and genotypes of the circulating MRSA clone isolated in bovine mastits milk samples in Xinjiang, China.
Fifty six MRSA isolates collected from milk of bovine mastitis were investigated by multilocus sequence typing (MLST), staphylococcal protein A (spa) typing, and a minimum inhibitory concentration test with 21 antimicrobial agents.
Antibiotic resistance results showed that 47.4% of the isolates were resistant to 16 or more antibiotics. Twelve MLST types were defined in this study, and ST398 (n = 7) and ST2393 (n = 2) were found to be the most prevalent types. Seven spa types (t034, t269, t4030, t114, t35, t189, and t7589) were identified, of which t034 (n = 7), t189 (n = 3), and t4030 (n = 3) were predominant. Here, 3 MRSA ST188 is reported among human MRSA isolates in China, and this is the first time that it is reported in bovine MRSA strains.
The antimicrobial susceptibility of MRSA in this area exhibited multidrug resistance, and clonal complexes CC398 and CC188, which have been reported among human MRSA isolates, do occur in Xinjiang dairy cows. This study provides a foundation for further MRSA monitoring.
Fifty six MRSA isolates collected from milk of bovine mastitis were investigated by multilocus sequence typing (MLST), staphylococcal protein A (spa) typing, and a minimum inhibitory concentration test with 21 antimicrobial agents.
Antibiotic resistance results showed that 47.4% of the isolates were resistant to 16 or more antibiotics. Twelve MLST types were defined in this study, and ST398 (n = 7) and ST2393 (n = 2) were found to be the most prevalent types. Seven spa types (t034, t269, t4030, t114, t35, t189, and t7589) were identified, of which t034 (n = 7), t189 (n = 3), and t4030 (n = 3) were predominant. Here, 3 MRSA ST188 is reported among human MRSA isolates in China, and this is the first time that it is reported in bovine MRSA strains.
The antimicrobial susceptibility of MRSA in this area exhibited multidrug resistance, and clonal complexes CC398 and CC188, which have been reported among human MRSA isolates, do occur in Xinjiang dairy cows. This study provides a foundation for further MRSA monitoring.
摘要：
由家畜相关耐甲氧西林金黄色葡萄球菌(MRSA)引起的疾病是一个重要的全球公共卫生问题。越来越多的人从牛乳中分离出MRSA。然而,新疆牛乳中MRSA的基因型和耐药性信息有限。本研究的目的是确定在新疆牛乳样品中分离的循环MRSA克隆的抗微生物敏感表型和基因型，中国。
通过多位点序列分型（MLST）研究了从牛乳腺炎乳中收集的56株MRSA分离株，葡萄球菌蛋白A（spa）分型，和21种抗菌剂的最低抑菌浓度试验。
抗生素耐药性结果显示，47.4%的分离株对16种以上抗生素耐药。本研究定义了12种MLST类型，发现ST398(n=7)和ST2393(n=2)是最普遍的类型。确定了七个水疗中心类型（t034、t269、t4030、t114、t35、t189和t7589），其中t034(n=7)，t189（n=3），t4030(n=3)占优势。这里,3MRSAST188在中国的人类MRSA分离株中报告，这是首次在牛MRSA菌株中报道。
该地区MRSA的抗菌药物敏感性表现出多重耐药性，和克隆复合物CC398和CC188，已在人类MRSA分离株中报道，确实发生在新疆奶牛身上。本研究为进一步的MRSA监测奠定了基础。
通过多位点序列分型（MLST）研究了从牛乳腺炎乳中收集的56株MRSA分离株，葡萄球菌蛋白A（spa）分型，和21种抗菌剂的最低抑菌浓度试验。
抗生素耐药性结果显示，47.4%的分离株对16种以上抗生素耐药。本研究定义了12种MLST类型，发现ST398(n=7)和ST2393(n=2)是最普遍的类型。确定了七个水疗中心类型（t034、t269、t4030、t114、t35、t189和t7589），其中t034(n=7)，t189（n=3），t4030(n=3)占优势。这里,3MRSAST188在中国的人类MRSA分离株中报告，这是首次在牛MRSA菌株中报道。
该地区MRSA的抗菌药物敏感性表现出多重耐药性，和克隆复合物CC398和CC188，已在人类MRSA分离株中报道，确实发生在新疆奶牛身上。本研究为进一步的MRSA监测奠定了基础。
