关键词: Auxis thazard Echinorhynchida Palaeacanthocephala cytochrome c oxidase subunit 1 (cox1) internal transcribed spacer (ITS) taxonomy

Mesh : Acanthocephala / classification genetics isolation & purification ultrastructure Animals China DNA, Helminth / chemistry genetics DNA, Ribosomal Spacer / chemistry genetics Electron Transport Complex IV / genetics Female Fish Diseases / parasitology Helminthiasis, Animal / parasitology Male Microscopy, Electron, Scanning / veterinary Mitochondrial Proteins / genetics Tuna / parasitology

来  源:   DOI:10.1515/ap-2018-0057

Abstract:
In the present study, Neorhadinorhynchus nudus (Harada, 1938) is reported from the frigate tuna Auxis thazard (Lacepéde) (Perciformes: Scombridae), in the South China Sea for the first time. The detailed morphology of N. nudus was studied using light and scanning electron microscopy based on the newly collected material. The results showed some morphometric variability between our specimens and previous studies, including the number of hooks per longitudinal row and the size of copulatory bursa and eggs. Our SEM observations also revealed all proboscis hooks emerged from elevated round rims on proboscis surface. In addition, N. nudus was firstly characterised using molecular methods by sequencing and analysing the ribosomal ITS and mitochondrial cox1 regions. There is no nucleotide divergence found in the ITS sequences, but a low level of nucleotide variability detected in the cox1 regions (the level of intraspecific nucleotide variability being 0.75% to 2.54%). The DNA sequence data obtained herein will indeed be a useful reference for rapid and accurate species identification of Neorhadinorhynchus.
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