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Abstract：
Oxidative stress and inflammation may contribute to anabolic resistance in response to protein and exercise in older adults. We investigated whether consumption of montmorency cherry concentrate (MCC) increased anabolic sensitivity to protein ingestion and resistance exercise in healthy older men.
Sixteen healthy older men were randomized to receive MCC (60 mL·d-1) or placebo (PLA) for two weeks, after baseline measures in week 1. During week 3, participants consumed 10 g whey protein·d-1 and completed three bouts of unilateral leg resistance exercise (4 × 8-10 repetitions at 80% 1RM). Participants consumed a bolus (150 mL) and weekly (50 mL) doses of deuterated water. Body water 2H enrichment was measured in saliva and vastus lateralis biopsies were taken from the non-exercised leg after weeks 1, 2 and 3, and the exercised leg after week 3, to measure tracer incorporation at rest, in response to protein and protein + exercise.
Myofibrillar protein synthesis increased in response to exercise + protein compared to rest (p < 0.05) in both groups, but there was no added effect of supplement (MCC: 1.79 ± 0.75 EX vs 1.15 ± 0.40 rest; PLA: 2.22 ± 0.54 vs 1.21 ± 0.18; all %·d-1). Muscle total NFĸB protein was decreased with exercise and protein in MCC (NFĸB: -20.7 ± 17.5%) but increased in PLA (NFĸB: 17.8 ± 31.3%, p = 0.073).
Short-term MCC ingestion does not affect the anabolic response to protein and exercise in healthy, relatively active, older men, despite MCC ingestion attenuating expression of proteins involved in the muscle inflammatory response to exercise, which may influence the chronic training response.
Sixteen healthy older men were randomized to receive MCC (60 mL·d-1) or placebo (PLA) for two weeks, after baseline measures in week 1. During week 3, participants consumed 10 g whey protein·d-1 and completed three bouts of unilateral leg resistance exercise (4 × 8-10 repetitions at 80% 1RM). Participants consumed a bolus (150 mL) and weekly (50 mL) doses of deuterated water. Body water 2H enrichment was measured in saliva and vastus lateralis biopsies were taken from the non-exercised leg after weeks 1, 2 and 3, and the exercised leg after week 3, to measure tracer incorporation at rest, in response to protein and protein + exercise.
Myofibrillar protein synthesis increased in response to exercise + protein compared to rest (p < 0.05) in both groups, but there was no added effect of supplement (MCC: 1.79 ± 0.75 EX vs 1.15 ± 0.40 rest; PLA: 2.22 ± 0.54 vs 1.21 ± 0.18; all %·d-1). Muscle total NFĸB protein was decreased with exercise and protein in MCC (NFĸB: -20.7 ± 17.5%) but increased in PLA (NFĸB: 17.8 ± 31.3%, p = 0.073).
Short-term MCC ingestion does not affect the anabolic response to protein and exercise in healthy, relatively active, older men, despite MCC ingestion attenuating expression of proteins involved in the muscle inflammatory response to exercise, which may influence the chronic training response.
摘要：
氧化应激和炎症可能导致老年人对蛋白质和运动的合成代谢抵抗。我们调查了食用montmorency樱桃浓缩物（MCC）是否会增加健康老年男性对蛋白质摄入和抵抗运动的合成代谢敏感性。
16名健康的老年男性随机接受MCC（60mL·d-1）或安慰剂（PLA）两周，基线测量后第1周。在第3周，参与者消耗了10g乳清蛋白·d-1，并完成了3次单侧腿部阻力运动（以80％1RM重复4×8-10次）。参与者消耗大丸剂(150mL)和每周(50mL)剂量的氘代水。在第1、2和3周之后，从非运动腿和第3周之后的运动腿进行唾液和股外侧肌活检，以测量休息时的示踪剂掺入，对蛋白质和蛋白质+运动的反应。
与休息相比，两组的肌原纤维蛋白合成均增加了对运动蛋白的反应（p<0.05），但没有补充的附加效应(MCC:1.79±0.75EXvs1.15±0.40休息;PLA:2.22±0.54vs1.21±0.18;所有%·d-1)。在MCC中，肌肉总NFkiB蛋白随着运动和蛋白质的增加而降低（NFB：-20.7±17.5％），但在PLA中增加（NFB：17.8±31.3％，p=0.073)。
短期MCC摄入不会影响健康人群对蛋白质和运动的合成代谢反应，相对活跃,年长的男人,尽管摄入MCC会减弱与运动引起的肌肉炎症反应有关的蛋白质的表达，这可能会影响慢性训练反应。
16名健康的老年男性随机接受MCC（60mL·d-1）或安慰剂（PLA）两周，基线测量后第1周。在第3周，参与者消耗了10g乳清蛋白·d-1，并完成了3次单侧腿部阻力运动（以80％1RM重复4×8-10次）。参与者消耗大丸剂(150mL)和每周(50mL)剂量的氘代水。在第1、2和3周之后，从非运动腿和第3周之后的运动腿进行唾液和股外侧肌活检，以测量休息时的示踪剂掺入，对蛋白质和蛋白质+运动的反应。
与休息相比，两组的肌原纤维蛋白合成均增加了对运动蛋白的反应（p<0.05），但没有补充的附加效应(MCC:1.79±0.75EXvs1.15±0.40休息;PLA:2.22±0.54vs1.21±0.18;所有%·d-1)。在MCC中，肌肉总NFkiB蛋白随着运动和蛋白质的增加而降低（NF
短期MCC摄入不会影响健康人群对蛋白质和运动的合成代谢反应，相对活跃,年长的男人,尽管摄入MCC会减弱与运动引起的肌肉炎症反应有关的蛋白质的表达，这可能会影响慢性训练反应。
