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Abstract：
Background: Diagnostic molecular marker studies are in vogue to have insight of most prevalent animal diseases including cancer. Objectives: Gene expression profiling of pro and anti-apoptotic genes was conducted in dog Lymphoma, CTVT, SCC, granuloma, perianal adenocarcinoma and mammary tumors. Materials and Methods: Cancerous tissues of 21 affected animals were obtained. Total RNA was extracted followed by cDNA synthesis. Comparative Ct method via Taqman assay (RT-qPCR) was used to quantify corresponding mRNA molecules, Tp53 and Hspb1, as normalized by GAPDH as the reference gene . Results:Hspb1 showed ectopic expression in lymphoma, CTVT and mammary tumors; its down-regulation was observed in granuloma and oral SCC with fold difference (FD) of ±35. Similarly, Tp53 as the tumor suppressor gene with pro-apoptotic properties, showed up-regulation in all tumor types, notably 80% of mammary tumors and 60% of CTVT. The FD values were 33.31 and 2.27, respectively. Conclusion: Altered transcriptomic response of Hspb1 and Tp53 was observed in all cancer types of Canis familiaris. The resulting profile depicts the involvement of the genes in cancer pathways. Thus, the data might be helpful for diagnosis, prognosis, identification and classification of these widespread neoplasms in this species.
摘要：
背景：诊断分子标记研究正在流行，以了解包括癌症在内的最常见的动物疾病。目的：在犬淋巴瘤中进行pro和抗凋亡基因的基因表达谱，CTVT,SCC,肉芽肿,肛周腺癌和乳腺肿瘤。材料和方法:获得21只患病动物的癌变组织。提取总RNA,然后进行cDNA合成。通过Taqman分析的比较Ct方法(RT-qPCR)用于定量相应的mRNA分子,Tp53和Hspb1,如通过作为参考基因的GAPDH标准化的。结果:Hspb1在淋巴瘤中呈异位表达,CTVT和乳腺肿瘤；在肉芽肿和口腔SCC中观察到其下调，差异倍数（FD）为±35。同样，Tp53作为具有促凋亡特性的抑癌基因，在所有肿瘤类型中都表现出上调，值得注意的是80%的乳腺肿瘤和60%的CTVT。FD值分别为33.31和2.27。结论：在犬的所有癌症类型中均观察到Hspb1和Tp53的转录组反应改变。所得到的谱描述了基因在癌症途径中的参与。因此，这些数据可能有助于诊断，预后,该物种中这些广泛存在的肿瘤的鉴定和分类。
