Abstract：
UNASSIGNED: To investigate the application value of the frozen-thawed round spermatids of the mouse in in vitro fertilization (IVF).
METHODS: Haploid spermatids of the mouse obtained in vitro were divided into a frozen-thawed and a fresh group and oocytes were collected from 6－8 weeks old female mice. After diamidino-phenyl-indole (DAPI) staining, the oocytes were subjected to intracytoplasmic round spermatid injection (ROSI), 259 in the frozen-thawed and 238 in the fresh group. Comparisons were made between the two groups in the capacities of fertilization and embryonic development.
RESULTS: The survival rate of the frozen-thawed haploid spermatids was (75.9 ± 2.3) %. No statistically significant differences were observed between the frozen-thawed and fresh groups after ROSI in the rates of fertilization (51.9 vs 55.7%, P >0.05), 2-cell embryos (51.0 vs 62.2%, P >0.05), 4－8-cell embryos (41.8 vs 42.9%, P >0.05), or morula-blastocysts (12.2 vs 21.4%, P >0.05).
CONCLUSIONS: Frozen-thawed round spermatids of the mouse are similar to fresh ones in their capacities of fertilization and embryonic development.
目的： 探讨冻融小鼠圆形精子细胞在体外受精中的应用价值。方法： 体外培养获得的小鼠单倍体精子细胞冻融复苏后，经 4\'，6-二眯基-2-苯基吲哚(DAPI)荧光染料标记后，行小鼠卵胞质内圆形精子细胞显微注射(ROSI) (冻融组，n=259)，同时以体外培养获得的新鲜单倍体精子细胞行ROSI (新鲜组，n=238)作对照，比较两组精子细胞受精能力和后续胚胎发育能力。结果： 体外培养获得的小鼠单倍体精子细胞冻融后存活率为(75.9±2.3)%。冻融组与新鲜组受精率(51.9% vs 55.7%)、2细胞率(51.0% vs 62.2%)、4~8细胞率(41.8% vs 42.9%)及桑囊胚形成率(12.2% vs 21.4%)均无显著性差异(P>0.05)。结论： 冻融复苏后的小鼠圆形精子细胞与冷冻前相似，具有一定的受精和胚胎发育能力。.
METHODS: Haploid spermatids of the mouse obtained in vitro were divided into a frozen-thawed and a fresh group and oocytes were collected from 6－8 weeks old female mice. After diamidino-phenyl-indole (DAPI) staining, the oocytes were subjected to intracytoplasmic round spermatid injection (ROSI), 259 in the frozen-thawed and 238 in the fresh group. Comparisons were made between the two groups in the capacities of fertilization and embryonic development.
RESULTS: The survival rate of the frozen-thawed haploid spermatids was (75.9 ± 2.3) %. No statistically significant differences were observed between the frozen-thawed and fresh groups after ROSI in the rates of fertilization (51.9 vs 55.7%, P >0.05), 2-cell embryos (51.0 vs 62.2%, P >0.05), 4－8-cell embryos (41.8 vs 42.9%, P >0.05), or morula-blastocysts (12.2 vs 21.4%, P >0.05).
CONCLUSIONS: Frozen-thawed round spermatids of the mouse are similar to fresh ones in their capacities of fertilization and embryonic development.
目的： 探讨冻融小鼠圆形精子细胞在体外受精中的应用价值。方法： 体外培养获得的小鼠单倍体精子细胞冻融复苏后，经 4\'，6-二眯基-2-苯基吲哚(DAPI)荧光染料标记后，行小鼠卵胞质内圆形精子细胞显微注射(ROSI) (冻融组，n=259)，同时以体外培养获得的新鲜单倍体精子细胞行ROSI (新鲜组，n=238)作对照，比较两组精子细胞受精能力和后续胚胎发育能力。结果： 体外培养获得的小鼠单倍体精子细胞冻融后存活率为(75.9±2.3)%。冻融组与新鲜组受精率(51.9% vs 55.7%)、2细胞率(51.0% vs 62.2%)、4~8细胞率(41.8% vs 42.9%)及桑囊胚形成率(12.2% vs 21.4%)均无显著性差异(P>0.05)。结论： 冻融复苏后的小鼠圆形精子细胞与冷冻前相似，具有一定的受精和胚胎发育能力。.
摘要：
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