{Reference Type}: Journal Article
{Title}: [Application value of frozen-thawed mouse round spermatids in in vitro fertilization].
{Author}: Jiang H;Wang L;Wang CL;Zhang WX;Ni F;
{Journal}: Zhonghua Nan Ke Xue
{Volume}: 23
{Issue}: 12
{Year}: Dec 2017
暂无{Abstract}: UNASSIGNED: To investigate the application value of the frozen-thawed round spermatids of the mouse in in vitro fertilization (IVF).
METHODS: Haploid spermatids of the mouse obtained in vitro were divided into a frozen-thawed and a fresh group and oocytes were collected from 6-8 weeks old female mice. After diamidino-phenyl-indole (DAPI) staining, the oocytes were subjected to intracytoplasmic round spermatid injection (ROSI), 259 in the frozen-thawed and 238 in the fresh group. Comparisons were made between the two groups in the capacities of fertilization and embryonic development.
RESULTS: The survival rate of the frozen-thawed haploid spermatids was (75.9 ± 2.3) %. No statistically significant differences were observed between the frozen-thawed and fresh groups after ROSI in the rates of fertilization (51.9 vs 55.7%, P >0.05), 2-cell embryos (51.0 vs 62.2%, P >0.05), 4-8-cell embryos (41.8 vs 42.9%, P >0.05), or morula-blastocysts (12.2 vs 21.4%, P >0.05).
CONCLUSIONS: Frozen-thawed round spermatids of the mouse are similar to fresh ones in their capacities of fertilization and embryonic development.
目的: 探讨冻融小鼠圆形精子细胞在体外受精中的应用价值。方法: 体外培养获得的小鼠单倍体精子细胞冻融复苏后,经 4',6-二眯基-2-苯基吲哚(DAPI)荧光染料标记后,行小鼠卵胞质内圆形精子细胞显微注射(ROSI) (冻融组,n=259),同时以体外培养获得的新鲜单倍体精子细胞行ROSI (新鲜组,n=238)作对照,比较两组精子细胞受精能力和后续胚胎发育能力。结果: 体外培养获得的小鼠单倍体精子细胞冻融后存活率为(75.9±2.3)%。冻融组与新鲜组受精率(51.9% vs 55.7%)、2细胞率(51.0% vs 62.2%)、4~8细胞率(41.8% vs 42.9%)及桑囊胚形成率(12.2% vs 21.4%)均无显著性差异(P>0.05)。结论: 冻融复苏后的小鼠圆形精子细胞与冷冻前相似,具有一定的受精和胚胎发育能力。.