其他低丰度人小核核糖核蛋白： U11 ， U12 等。Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.-医云文献数字医云科研云海量医学决策数据服务

Abstract：

Two-dimensional gel fractionation has revealed the existence of a number (greater than or equal to 8) of additional species of HeLa cell small RNAs that have 5\' trimethylguanosine cap structures and are bound by proteins containing Sm epitopes. Therefore, these low-abundance (10(3)-10(4) per cell) RNAs belong to the Sm class of small nuclear ribonucleoproteins (snRNPs), whose best-known members are the four highly abundant (approximately 10(6) per cell) particles required for pre-mRNA splicing. The complexity of Sm snRNPs in mammalian cells is thus not greatly different from that previously established for lower eukaryotes. Two of the new RNAs, designated U11 (131 nucleotides) and U12 (150 nucleotides), have been sequenced. The U11 and U12 snRNPs have been characterized further by examining their nuclease sensitivity and their possible interactions with other snRNPs. Potential roles for the low-abundance snRNPs in aspects of pre-mRNA processing are discussed.

摘要：

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Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.

1 A 64 kd nuclear protein binds to RNA segments that include the AAUAAA polyadenylation motif.

2 Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylation.

3 Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's.

4 Synthesis of small nuclear ribonucleoprotein particles by the malarial parasite Plasmodium falciparum.

5 A protein that specifically recognizes the 3' splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoprotein.

6 Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.

7 The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.

8 Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.

9 Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.

10 Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity.

11 High level of complexity of small nuclear RNAs in fungi and plants.

12 Two proteins crosslinked to RNA containing the adenovirus L3 poly(A) site require the AAUAAA sequence for binding.

13 A protein associated with small nuclear ribonucleoprotein particles recognizes the 3' splice site of premessenger RNA.

14 Primary and secondary structure of U8 small nuclear RNA.

15 Requirements for accurate and efficient mRNA 3' end cleavage and polyadenylation of a simian virus 40 early pre-RNA in vitro.

16 A new method for sequencing DNA.

17 3'-terminal labelling of RNA with T4 RNA ligase.

18 Mapping adenines, guanines, and pyrimidines in RNA.

19 Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.

20 A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro.

21 Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.

22 U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.

23 Specific contacts between mammalian U7 snRNA and histone precursor RNA are indispensable for the in vitro 3' RNA processing reaction.

24 Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNA.

25 Multiple factors are required for specific RNA cleavage at a poly(A) addition site.

26 Plant small nuclear RNAs. Nucleolar U3 snRNA is present in plants: partial characterization.

27 Autoantibodies to the U2 small nuclear ribonucleoprotein in a patient with scleroderma-polymyositis overlap syndrome.

28 U1, U2 and U5 small nuclear RNAs are found in plants cells. Complete nucleotide sequence of the U5 RNA family from pea nuclei.

29 Yeast contains small nuclear RNAs encoded by single copy genes.

30 Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein.

31 Transcription termination and 3' processing: the end is in site!

32 Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis.

33 The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing.

34 3' cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNP.

35 Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particles.