关键词: Biogenic amines HPLC Mass spectrometry Mycotoxins NMR metabolite profile Red sweet pepper α-Amylase inhibition

Mesh : Antifungal Agents / pharmacology Biogenic Amines / analysis Capsicum / chemistry metabolism Chromatography, High Pressure Liquid Flavonoids / analysis Food Analysis / methods Food Quality Fruit / chemistry Magnetic Resonance Spectroscopy Mass Spectrometry Mycotoxins / analysis Plant Extracts / pharmacology Polyphenols / analysis Seeds / chemistry Tannins / analysis alpha-Amylases / antagonists & inhibitors

来  源:   DOI:10.1016/j.foodchem.2018.02.050

Abstract:
A multi-methodological approach was applied to study red sweet peppers (Capsicum annuum L.) ecotype \"Cornetto di Pontecorvo\" grown in a greenhouse or in open field. This approach includes morphological analysis, chemical composition determination, and biological activity evaluation of different extracts from pepper fruits. Untargeted analyses, namely NMR spectroscopy and mass spectrometry, allowed the comprehensive pepper metabolite profile of pepper pulp, peel and seeds hydroalcoholic and organic extracts to be determined, showing the presence of sugars, organic acids, amino acids and other secondary metabolites. Targeted analyses, such as HPLC-PDA, HPLC-TLC and spectrophotometric analyses allowed polyphenols, tannins, flavonoids and pigments content to be determined. Samples quality and freshness were verified by the low content of biogenic amines and mycotoxins, as determined using HPLC-FLD and HPLC-MS, respectively. Preliminary biological results demonstrated the ability of the organic extracts to inhibit α-amylase, a key enzyme in the control of glucose metabolism.
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