关键词: Alkaline extraction Alkaline hydrogen peroxide Bioethanol Enzymatic hydrolysis Simultaneous saccharification and co-fermentation Wheat straw

Mesh : Ethanol Fermentation Hydrogen Peroxide Hydrolysis Lignin Triticum

来  源:   DOI:10.1016/j.biortech.2018.03.044

Abstract:
An efficient scheme was developed for the conversion of wheat straw (WS) into bioethanol, silica and lignin. WS was pre-extracted with 0.2 mol/L sodium hydroxide at 30 °C for 5 h to remove about 91% of initial silica. Subsequently, the alkaline-pretreated solids were subjected to alkaline hydrogen peroxide (AHP) pretreatment with 40 mg hydrogen peroxide (H2O2)/g biomass at 50 °C for 7 h to prepare highly digestible substrate. The results of enzymatic hydrolysis demonstrated that the sequential alkaline-AHP pretreated WS was efficiently hydrolyzed at 10% (w/v) solids loading using an enzyme dosage of 10 mg protein/g glucan. The total sugar conversion of 92.4% was achieved. Simultaneous saccharification and co-fermentation (SSCF) was applied to produce ethanol from the two-stage pretreated substrate using Saccharomyces cerevisiae SR8u strain. Ethanol with concentration of 31.1 g/L was produced. Through the proposed process, about 86.4% and 54.1% of the initial silica and lignin were recovered, respectively.
摘要:
开发了一种将小麦秸秆(WS)转化为生物乙醇的有效方案,二氧化硅和木质素。在30°C下用0.2mol/L氢氧化钠预萃取WS5小时以除去约91%的初始二氧化硅。随后,将碱预处理的固体在50°C下用40mg过氧化氢(H2O2)/g生物质进行碱性过氧化氢(AHP)预处理7小时,以制备高度易消化的底物。酶促水解的结果表明,使用10mg蛋白质/g葡聚糖的酶剂量,连续的碱性AHP预处理的WS在10%(w/v)固体负载下有效水解。实现了92.4%的总糖转化率。同时糖化和共发酵(SSCF)用于使用酿酒酵母SR8u菌株从两阶段预处理的底物生产乙醇。产生浓度为31.1g/L的乙醇。经由过程建议,回收了约86.4%和54.1%的初始二氧化硅和木质素,分别。
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