RESULTS: The results showed a mixed-mode mechanism comprising of hydrophilic (partition) and electrostatic interaction (ion exchange) for MET and essentially hydrophilic for TEN. The linear calibration curves were established in the concentration range of 1.0-1000 ng/ml for MET and 0.50-750 ng/ml for TEN.
CONCLUSIONS: The method was applied to determine plasma concentration of MET and TEN in healthy subjects.