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Abstract：
Whole-cell biocatalysts offer a highly enantioselective, minimally polluting route to optically active alcohols. Currently, most of the whole-cell catalytic performance involves resting cells rather than growing cell biotransformation, which is one-step process that benefits from the simultaneous growth and biotransformation, eliminating the need for catalysts preparation. In this paper, asymmetric reduction of 14 aromatic ketones to the corresponding enantiomerically pure alcohols was successfully conducted using the growing and resting cells of marine-derived fungi under optimized conditions. Good yields and excellent enantioselectivities were achieved with both methods. Although substrate inhibition might be a limiting factor for growing cell biotransformation, the selected strain can still completely convert 10-mM substrates into the desired products. The resting cell biotransformation showed a capacity to be recycled nine times without a significant decrease in the activity. This is the first study to perform asymmetric reduction of ketones by one-step growing cell biotransformation.
摘要：
全细胞生物催化剂提供了高度的对映选择性，对光学活性醇的污染最小的途径。目前,大多数全细胞催化性能涉及静止细胞而不是生长细胞生物转化，这是一步过程，受益于同时生长和生物转化，消除了催化剂制备的需要。在本文中,在优化条件下，使用海洋真菌的生长和静止细胞成功地将14个芳族酮不对称还原为相应的对映体纯醇。两种方法均获得了良好的产率和优异的对映选择性。尽管底物抑制可能是生长细胞生物转化的限制因素，所选择的菌株仍可将10-mM底物完全转化为所需产物。静息细胞生物转化显示出可循环九次的能力，而活性却没有显着降低。这是第一项通过一步生长细胞生物转化进行酮不对称还原的研究。
