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Abstract：
The tree shrew, as a primate-like animal model, has been used for studying high brain functions such as social emotion and spatial learning memory. However, little is known about the excitatory synaptic transmission in cortical brain areas of the tree shrew. In the present study, we have characterized the excitatory synaptic transmission and intrinsic properties of pyramidal neurons in the anterior cingulate cortex (ACC) of the adult tree shrew, a key cortical region for pain perception and emotion. We found that glutamate is the major excitatory transmitter for fast synaptic transmission. Excitatory synaptic responses induced by local stimulation were mediated by AMPA and kainate (KA) receptors. As compared with mice, AMPA and KA receptor mediated responses were significantly greater. Interestingly, the frequency of spontaneous excitatory postsynaptic currents (sEPSCs) and miniature excitatory postsynaptic currents (mEPSCs) in tree shrews was significantly less than that of mice. Moreover, both the ratio of paired-pulse facilitation (PPF) and the time of 50% decay for fast blockade of NMDA receptor mediated EPSCs were greater in the tree shrew. Finally, tree shrew neurons showed higher initial firing frequency and neuronal excitability with a cell type-specific manner in the ACC. Our studies provide the first report of the basal synaptic transmission in the ACC of adult tree shrew.
摘要：
树泼妇，作为灵长类动物的模型,已被用于研究高大脑功能，如社会情绪和空间学习记忆。然而,关于树的大脑皮层区的兴奋性突触传递知之甚少。在本研究中,我们已经描述了成年树的前扣带皮质（ACC）中锥体神经元的兴奋性突触传递和内在特性，疼痛感知和情绪的关键皮质区域。我们发现谷氨酸是快速突触传递的主要兴奋性递质。局部刺激诱导的兴奋性突触反应由AMPA和红藻氨酸（KA）受体介导。与老鼠相比，AMPA和KA受体介导的反响显著较年夜。有趣的是,树the的自发兴奋性突触后电流（sEPSCs）和微型兴奋性突触后电流（mEPSCs）的频率显着低于小鼠。此外，双脉冲促进(PPF)的比率和快速阻断NMDA受体介导的EPSC的50%衰减时间在树the中更大.最后,树the神经元在ACC中表现出更高的初始放电频率和神经元兴奋性，具有细胞类型特异性。我们的研究提供了成年树theACC中基础突触传递的第一份报告。
