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Abstract：
Stress granules are mRNA-protein assemblies formed from nontranslating mRNAs. Stress granules are important in the stress response and may contribute to some degenerative diseases. Here, we describe the stress granule transcriptome of yeast and mammalian cells through RNA-sequencing (RNA-seq) analysis of purified stress granule cores and single-molecule fluorescence in situ hybridization (smFISH) validation. While essentially every mRNA, and some noncoding RNAs (ncRNAs), can be targeted to stress granules, the targeting efficiency varies from <1% to >95%. mRNA accumulation in stress granules correlates with longer coding and UTR regions and poor translatability. Quantifying the RNA-seq analysis by smFISH reveals that only 10% of bulk mRNA molecules accumulate in mammalian stress granules and that only 185 genes have more than 50% of their mRNA molecules in stress granules. These results suggest that stress granules may not represent a specific biological program of messenger ribonucleoprotein (mRNP) assembly, but instead form by condensation of nontranslating mRNPs in proportion to their length and lack of association with ribosomes.
摘要：
应激颗粒是由非翻译mRNA形成的mRNA-蛋白质组装体。应激颗粒在应激反应中很重要，可能会导致一些退行性疾病。这里,我们通过纯化的应激颗粒核心的RNA测序(RNA-seq)分析和单分子荧光原位杂交(smFISH)验证,描述了酵母和哺乳动物细胞的应激颗粒转录组.虽然基本上每个mRNA，和一些非编码RNA（ncRNAs），可以针对应力颗粒，靶向效率从<1%到>95%不等。应激颗粒中的mRNA积累与更长的编码区和UTR区以及较差的可翻译性相关。通过smFISH对RNA-seq分析进行定量表明，只有10％的本体mRNA分子在哺乳动物应激颗粒中积累，并且只有185个基因在应激颗粒中具有超过50％的mRNA分子。这些结果表明，应激颗粒可能不代表信使核糖核蛋白（mRNP）组装的特定生物学程序，而是通过与长度成比例的非翻译mRNPs缩合而形成，并且缺乏与核糖体的缔合。
