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Abstract：
Seriola lalandi is an economically important species that is globally distributed in temperate and subtropical marine waters. Aquaculture production of this species has had problems associated with intensive fish farming, such as disease outbreaks or nutritional deficiencies causing high mortalities. Intestinal microbiota has been involved in many processes that benefit the host, such as disease control, stimulation of the immune response, and the promotion of nutrient metabolism, among others. However, little is known about the potential functionality of the microbiota and the differences in the composition between wild and aquacultured fish. Here, we assayed the V4-region of the 16S rRNA gene using high-throughput sequencing. Our results showed that there are significant differences between S. lalandi of wild and aquaculture origin (ANOSIM and PERMANOVA, P < 0.05). At the genus level, a total of 13 genera were differentially represented between the two groups, all of which have been described as beneficial microorganisms that have an antagonistic effect against pathogenic bacteria, improve immunological parameters and growth performance, and contribute to nutrition. Additionally, the changes in the presumptive functions of the intestinal microbiota of yellowtail were examined by predicting the metagenomes using PICRUSt. The most abundant functional categories were those corresponding to the metabolism of cofactors and vitamins, amino acid metabolism and carbohydrate metabolism, revealing differences in the contribution of the microbiota depending on the origin of the animals. To our knowledge, this is the first study to characterize and compare the intestinal microbiota of S. lalandi of wild and aquaculture origin using high-throughput sequencing.
摘要：
Seriolalalandi是一种重要的经济物种，在全球范围内分布在温带和亚热带海洋水域。该物种的水产养殖生产存在与集约化鱼类养殖相关的问题，如疾病爆发或营养缺乏导致高死亡率。肠道微生物群参与了许多有益于宿主的过程，比如疾病控制，刺激免疫反应，促进营养代谢，在其他人中。然而,关于微生物群的潜在功能以及野生和水产养殖鱼类之间的组成差异知之甚少。这里,我们使用高通量测序检测了16SrRNA基因的V4区.我们的结果表明，野生和水产养殖的S.lalandi之间存在显着差异（ANOSIM和PERMANOVA，P<0.05)。在属一级，两组共有13个属有差异，所有这些都被描述为对病原菌具有拮抗作用的有益微生物，提高免疫学参数和生长性能，并有助于营养。此外,通过使用PICRUSt预测宏基因组来检查黄鱼肠道微生物群的推定功能的变化。最丰富的功能类别是与辅因子和维生素的代谢相对应的那些，氨基酸代谢和碳水化合物代谢，揭示微生物群贡献的差异取决于动物的起源。据我们所知,这是第一项使用高通量测序技术表征和比较野生和水产养殖来源的拉兰地肠道微生物群的研究.
