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Abstract：
Acanthamoeba culbertsoni trophozoites, previously isolated from a human keratitis case with severe intraocular damage, were maintained in axenic culture. Co-incubation of amoebae with MDCK cell monolayers demonstrated an apparent preference of the amoebae to introduce themselves between the cells. The trophozoites appeared to cross the cell monolayer through the tight junctions, which resulted in decreased trans-epithelial resistance (TER) measurements. Unexpectedly, after co-incubation of amoebae with hamster corneas, we observed that the trophozoites were able to cross the different cell layers and reach the corneal stroma after only 12 h of interaction, in contrast to other Acanthamoeba species. These observations suggest that this A. culbertsoni isolate is particularly pathogenic. Further research with diverse methodologies needs to be performed to explain the unique behavior of this Acanthamoeba strain.
摘要：
棘阿米巴滋养体，先前从患有严重眼内损伤的人类角膜炎病例中分离出来，保持在无菌培养中。变形虫与MDCK细胞单层的共孵育表明变形虫明显倾向于在细胞之间引入自身。滋养体似乎通过紧密连接穿过细胞单层，这导致减少的跨上皮阻力（TER）测量。出乎意料的是,变形虫与仓鼠角膜共同孵化后，我们观察到滋养体能够穿过不同的细胞层，并在仅12小时的相互作用后到达角膜基质，与其他棘阿米巴物种相反。这些观察结果表明，这种A.culbertsoni分离株特别具有致病性。需要进行各种方法的进一步研究，以解释这种棘阿米巴菌株的独特行为。
